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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Balan, Venkatesh Ladisch, Michael R. Ebrik, Mirvat A. Holtzapple, Mark T. Mosier, Nathan S. Falls, Matthew Shi, Jian Dale, Bruce E. Wyman, Charles E. Elander, Richard T. Lee, Y. Y. Warner, Ryan E. Garlock, Rebecca J. Kim, Youngmi Donohoe, Bryon S. Vinzant, Todd B. Hames, Bonnie Thomas, Steve Yang, Bin Pallapolu, Venkata Ramesh Sierra-Ramirez, Rocio Redmond, Tim |
| Description | Country affiliation: United States Author Affiliation: Pallapolu VR ( Department of Chemical Engineering, Auburn University, 212 Ross Hall, Auburn, AL 36849, USA.) |
| Abstract | The objective of this work is to investigate the effects of cellulase loading and ß-glucosidase supplementation on enzymatic hydrolysis of pretreated Dacotah switchgrass. To assess the difference among various pretreatment methods, the profiles of sugars and intermediates were determined for differently treated substrates. For all pretreatments, 72 h glucan/xylan digestibilities increased sharply with enzyme loading up to 25mg protein/g-glucan, after which the response varied depending on the pretreatment method. For a fixed level of enzyme loading, dilute sulfuric acid (DA), SO(2), and Lime pretreatments exhibited higher digestibility than the soaking in aqueous ammonia (SAA) and ammonia fiber expansion (AFEX). Supplementation of Novozyme-188 to Spezyme-CP improved the 72 h glucan digestibility only for the SAA treated samples. The effect of ß-glucosidase supplementation was discernible only at the early phase of hydrolysis where accumulation of cellobiose and oligomers is significant. Addition of ß-glucosidase increased the xylan digestibility of alkaline treated samples due to the ß-xylosidase activity present in Novozyme-188. |
| ISSN | 09608524 |
| Issue Number | 24 |
| Volume Number | 102 |
| e-ISSN | 18732976 |
| Journal | Bioresource Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-12-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biotechnology Methods Panicum Metabolism Beta-glucosidase Cellobiose Glucans Glucose Hydrolysis Drug Effects Journal Article Research Support, U.s. Gov't, Non-p.h.s. Discipline Bioresource |
| Content Type | Text |
| Resource Type | Article |
| Subject | Waste Management and Disposal Medicine Environmental Engineering Renewable Energy, Sustainability and the Environment Bioengineering |
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