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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhu, Lingqing Yang, Xue Qu, Liang Chen, Yu Xue, Chaoyou Lu, Wenyu |
| Description | Country affiliation: China Author Affiliation: Zhu L ( Department of Biological Engineering and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.) |
| Abstract | Rhamnolipids find broad applications as natural surfactants, emulsifiers, and antibiotics because of their low toxicity, high biodegradability and environmental soundness. In this study, a pH stage-controlled process of fermentation of rhamnolipids by Pseudomonas aeruginosa O-2-2 was established. A yield of 24.06 g/L in batch fermentation was achieved in a 5-L fermentor via the optimization of stirring speed. By controlling pH, rhamnolipid production was increased to 28.8 g/L, an improvement of 19.7%, and more substrate was converted to rhamnolipids rather than to biomass. Fermentation kinetics models for cell growth, product synthesis and substrate consumption based on the pH stage-controlled fermentation indicated that rhamnolipid production could be further improved by fed-batch fermentation. Rhamnolipid production reached 70.56 g/L, an improvement of 193%, in the pH stage-controlled fed-batch fermentation when the stirring speeds was controlled at 500 rpm and the fermentation temperature was maintained at 30 °C. |
| ISSN | 09608524 |
| Volume Number | 117 |
| e-ISSN | 18732976 |
| Journal | Bioresource Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-08-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Batch Cell Culture Techniques Methods Fermentation Physiology Glycolipids Biosynthesis Pseudomonas Aeruginosa Metabolism Biomass Hydrogen-ion Concentration Kinetics Models, Biological Oxygen Growth & Development Solubility Journal Article Research Support, Non-u.s. Gov't Discipline Bioresource |
| Content Type | Text |
| Resource Type | Article |
| Subject | Waste Management and Disposal Medicine Environmental Engineering Renewable Energy, Sustainability and the Environment Bioengineering |
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