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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Li, Xiang Zhao, Yuandi Liu, Tao Wang, Xiaofeng Yan, Yunjun |
| Description | Author Affiliation: Liu T ( Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.) |
| Abstract | A novel oriented immobilized lipase was derived from Yarrowia lipolytica lipase LIP2 covalently immobilized on functionalized Fe3O4 magnetic nanoparticles (MNPs) in reverse micelles system (RMS). The activity recovery reached 382% compared with 29% in aqueous phase, and further ran up to 1425% under optimum conditions. (3-Aminopropyl) triethoxysilane (APTES) coated Fe3O4 nanoparticles were characterized by Fourier transform infrared (FT-IR) and X-ray diffraction (XRD). A significant alteration in the secondary structure of the lipase in RMS with a 15.5% increase of -helix content and a 12.5% decrease of ß-sheet content was detected by circular dichroism (CD). The immobilized lipase was employed to enrich polyunsaturated fatty acids in fish oil, a 90% increase of DHA content was obtained after 12h, and after 20 cycles of successive usage, it still remained over 80% of relative hydrolysis degree, which shows a good recyclability. |
| ISSN | 09608524 |
| Volume Number | 132 |
| e-ISSN | 18732976 |
| Journal | Bioresource Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-03-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Enzymes, Immobilized Metabolism Fatty Acids, Unsaturated Lipase Magnetite Nanoparticles Chemistry Circular Dichroism Micelles Propylamines Protein Conformation Silanes Spectroscopy, Fourier Transform Infrared X-ray Diffraction Yarrowia Enzymology Journal Article Research Support, Non-u.s. Gov't Discipline Bioresource |
| Content Type | Text |
| Resource Type | Article |
| Subject | Waste Management and Disposal Medicine Environmental Engineering Renewable Energy, Sustainability and the Environment Bioengineering |
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