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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Loncar, Nikola Bozic, Natasa Vujcic, Zoran Lopez-Santin, Josep |
| Description | Author Affiliation: Loncar N ( Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, Belgrade, Serbia. Electronic address: nloncar@chem.bg.ac.rs.) |
| Abstract | One hundred wild type strains of Bacillus sp. were isolated from industrial and agricultural soil across Serbia and screened for laccase activity. Three strains showed high laccase activity temperature optimum of 65 and 80 °C towards ABTS. A new laccase gene from the strain with highest temperature optimum, namely Bacillus amyloliquefaciens 12B was cloned and expressed in Escherichia coli. Recombinant laccase degraded dye Reactive blue 52 at pH 7.0 and pH 4.0 and at elevated temperature, while fungal laccases was unable to act on this substrate at pH higher than 4.0 and was quickly inactivated at temperatures higher than 45 °C. Degradation of dye was monitored by HPLC-DAD and resulting precipitate was analyzed by FTIR spectroscopy. Single product peak without chromophore was detected in solution, while water insoluble aggregate, presumably dye polymer is formed retaining blue color. |
| ISSN | 09608524 |
| Volume Number | 147 |
| e-ISSN | 18732976 |
| Journal | Bioresource Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-11-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Bacillus Enzymology Color Laccase Metabolism Soil Microbiology Waste Water Water Purification Methods Amino Acid Sequence Chromatography, High Pressure Liquid Chemistry Genetics Molecular Sequence Data Recombinant Proteins Sequence Homology, Amino Acid Spectroscopy, Fourier Transform Infrared Journal Article Research Support, Non-u.s. Gov't Discipline Bioresource |
| Content Type | Text |
| Resource Type | Article |
| Subject | Waste Management and Disposal Medicine Environmental Engineering Renewable Energy, Sustainability and the Environment Bioengineering |
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