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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Martins, Samuel Gois, Pedro M. P. Ribeiro, Maria H. L. Fernandes, Pedro C. B. Nunes, Mário A. P. Rosa, M. Emilia |
| Description | Country affiliation: Portugal Author Affiliation: Nunes MA ( Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia, Universidade Lisboa, Av. Gama Pinto, 1649-003 Lisboa, Portugal.); Martins S ( Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia, Universidade Lisboa, Av. Gama Pinto, 1649-003 Lisboa, Portugal.); Rosa ME ( Instituto de Ciência e Engenharia de Materiais e Superfícies, Departamento de Engenharia de Materiais, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.); Gois PM ( Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia, Universidade Lisboa, Av. Gama Pinto, 1649-003 Lisboa, Portugal.); Fernandes PC ( Institute of Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.); Ribeiro MH ( Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia, Universidade Lisboa, Av. Gama Pinto, 1649-003 Lisboa, Portugal. Electronic address: mhribeiro@ff.ulisboa.pt.) |
| Abstract | Polyvinyl alcohol (PVA) electrospun nanofibers were produced using an electrospinning technique. Key parameters (e.g. collectors, distance from needle tip to collector, among others) that influence the structure and morphology of fibers were optimized. The naringinase entrapped in PVA nanofibers retained over 100% of its initial activity after 212h of operation, at 25°C. Chemical crosslinking with several boronic acids further increased the hydrolysis temperature (up to 85°C) and yielded nanofibers with thermal stability up to 121°C. A mini packed bed reactor (PBR) developed to establish the feasibility for continuous enzymatic operation, ran for 16days at 45°C. Highest naringenin biosynthesis was attained at a flow rate of 10mLh(-1). Highest volumetric (78molL(-1)h(-1)) and specific (26molh(-1)genzyme(-1)) productivities were attained at 30mLh(-1). The activity of NGase in electrospun nanofibers remained constant for almost 16days of operation at 10mLh(-1). |
| ISSN | 09608524 |
| Journal | Bioresource Technology |
| Volume Number | 213 |
| e-ISSN | 18732976 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-08-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Bioresource |
| Content Type | Text |
| Resource Type | Article |
| Subject | Waste Management and Disposal Medicine Environmental Engineering Renewable Energy, Sustainability and the Environment Bioengineering |
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