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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Liu, Jingjing Yuan, Yingjin Zhao, Fanglong Du, Yanhui Bai, Peng Lu, Wenyu |
| Description | Author Affiliation: Zhao F ( School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, PR China.); Du Y ( School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, PR China.); Bai P ( School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, PR China); Liu J ( School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, PR China.); Lu W ( School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, PR China); Yuan Y ( School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, PR China) |
| Abstract | Protopanaxadiol (PPD) is an active compound in Panax ginseng. Recently, an optimized PPD synthesis pathway contained a ROS releasing step (a P450-type PPD synthase, PPDS) was introduced into Saccharomyces cerevisiae. Here reported a synergistic effect of PPDS-CPR (CPR, cytochrome P450 reductase) uncoupling and ethanol stress on ROS releasing, which reduced cells viability. To build a robust strain, a cell wall integrity associated gene SSD1 was high-expressed to improve ethanol tolerance, and ROS level decreased for 24.7%. Then, regulating the expression of an oxidative stress regulation gene YBP1 decreased 75.2% of ROS releasing, and improved cells viability from 71.3±1.3% to 88.3±1.4% at 84h. Increased cells viability enables yeast to produce more PPD through feeding additional ethanol. In 5L fermenter, PPD production of W3a-ssPy reached to 4.25±0.18g/L (19.48±0.28mg/L/OD600), which is the highest yield reported so far. This work makes the industrial production of PPD possible by microbial fermentation. |
| ISSN | 09608524 |
| Journal | Bioresource Technology |
| Volume Number | 227 |
| e-ISSN | 18732976 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2017-03-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Fermentation Saccharomyces Cerevisiae Genetics Metabolism Sapogenins Chemical Synthesis Reactive Oxygen Species Saccharomyces Cerevisiae Proteins Journal Article Discipline Bioresource |
| Content Type | Text |
| Resource Type | Article |
| Subject | Waste Management and Disposal Medicine Environmental Engineering Renewable Energy, Sustainability and the Environment Bioengineering |
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