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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Freed, Jack H. Tombolato, Fabio Ferrarini, Alberta |
| Description | Author Affiliation: Tombolato F ( Dipartimento di Scienze Chimiche, Via Marzolo 1, Università di Padova, 35131 Padova, Italy.) |
| Abstract | In the companion paper (J. Phys. Chem. B 2006, 110, jp0629487), a study of the conformational dynamics of methanethiosulfonate spin probes linked at a surface-exposed alpha-helix has been presented. Here, on the basis of this analysis, X-band ESR spectra of these spin labels are simulated within the framework of the Stochastic Liouville equation (SLE) methodology. Slow reorientations of the whole protein are superimposed on fast chain motions, which have been identified with conformational jumps and fluctuations in the minima of the chain torsional potential. Fast chain motions are introduced in the SLE for the protein reorientations through partially averaged magnetic tensors and relaxation times calculated according to the motional narrowing theory. The 72R1 and 72R2 mutants of T4 lysozyme, which bear the spin label at a solvent-exposed helix site, have been taken as test systems. For the side chain of the R2 spin label, only a few noninterconverting conformers are possible, whose mobility is limited to torsional fluctuations, yielding almost identical spectra, typical of slightly mobile nitroxides. In the case of R1, more complex spectra result from the simultaneous presence of constrained and mobile chain conformers, with relative weights that can depend on the local environment. The model provides an explanation for the experimentally observed dependence of the spectral line shapes on temperature, solvent, and pattern of substituents in the pyrroline ring. The relatively simple methodology presented here allows the introduction of realistic features of the spin probe dynamics into the simulation of ESR spectra of spin-labeled proteins; moreover, it provides suggestions for a proper account of such dynamics in more sophisticated approaches. |
| ISSN | 15206106 |
| e-ISSN | 15205207 |
| Journal | The Journal of Physical Chemistry B |
| Issue Number | 51 |
| Volume Number | 110 |
| Language | English |
| Publisher | American Chemical Society (United States) |
| Publisher Date | 2006-12-28 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Nitrogen Oxides Chemistry Proteins Electron Spin Resonance Spectroscopy Models, Molecular Spin Labels Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Physical chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Surfaces, Coatings and Films Materials Chemistry Medicine Physical and Theoretical Chemistry |
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