NDLI: Kinetic and spectroscopic evidence of negative cooperativity in the action of lysine 2,3-aminomutase.

Please wait, while we are loading the content...

Year: 2017 Volume: 121

Year: 2016 Volume: 120

Year: 2015 Volume: 119

Year: 2014 Volume: 118

Year: 2013 Volume: 117

Year: 2012 Volume: 116

Year: 2011 Volume: 115

Year: 2010 Volume: 114

Year: 2010 Volume: 114 Issue: 51

Year: 2010 Volume: 114 Issue: 50

Year: 2010 Volume: 114 Issue: 49

Thermodynamics of the Hydrolysis Reactions of 1-Naphthyl Acetate, 4-Nitrophenyl Acetate, and 4-Nitrophenyl $α-l-arabinofuranoside^{†}$

A database of thermodynamic quantities for the reactions of glycolysis and the tricarboxylic acid cycle.

Consumption of Hydrogen Ions in Rapid-Equilibrium Enzyme $Kinetics^{†}$

Kinetic and spectroscopic evidence of negative cooperativity in the action of lysine 2,3-aminomutase.

Thermodynamic Origin of Selective Binding of β-Cyclodextrin Derivatives with Chiral Chromophoric Substituents toward $Steroids^{†}$

Precise, facile initial rate measurements.

The Role of $Zn^{2+}$ on the Structure and Stability of Murine Adenosine Deaminase

Pressure Perturbation Calorimetry of Unfolded $Proteins^{†}$

Experiment and Prediction: A Productive Symbiosis in Studies on the Thermodynamics of DNA $Oligomers^{†}$

Cyclic Conformational Modification of an Enzyme: Serial Engagement, Energy Relay, Hysteretic Enzyme, and Fischer’s $Hypothesis^{†}$

The Effect of pH and Free $Mg^{2+}$ on ATP Linked Enzymes and the Calculation of Gibbs Free Energy of ATP $Hydrolysis^{†}$

Pressure Perturbation Calorimetry and the Thermodynamics of Noncovalent Interactions in Water: Comparison of Protein−Protein, Protein−Ligand, and Cyclodextrin−Adamantane $Complexes^{†}$

Conformational dynamics in human purine nucleoside phosphorylase with reactants and transition-state analogues.

Cationic Polyelectrolytes as Drug Delivery Vectors: Calorimetric and Fluorescence Study of Rutin $Partitioning^{†}$

Influence of Lysine $N^{ε}-Trimethylation$ and Lipid Composition on the Membrane Activity of the Cecropin A-Melittin Hybrid Peptide $CA(1−7)M(2−9)^{†}$

Energetics and Molecular Structure of 2,5-Dimethyl-1-phenylpyrrole and $2,5-Dimethyl-1-(4-nitrophenyl)pyrrole^{†}$

Entropy, Information, and the Arrow of $Time^{†}$

Desolvation Penalty for Burying Hydrogen-Bonded Peptide Groups in Protein $Folding^{†}$

Measurement of Nanoparticle Concentration Using Quartz Crystal $Microgravimetry^{†}$

Disparate Impact of the S33V Mutation on Conformational Stability in Rat β-Parvalbumin (Oncomodulin) and Chicken Parvalbumin $3^{†}$

Optimal Efficiency of Self-Assembling Light-Harvesting $Arrays^{†}$

Kinetic and Thermodynamic Aspects of Enzyme Control and $Regulation^{†}$

Energetics of the Lighter Chalcogen Analogues of Carboxylic Acid $Esters^{†}$

Specificity of Non-Michaelis−Menten Enzymes: Necessary Information for Analyzing Metabolic $Pathways^{†}$

Parameter Balancing in Kinetic Models of Cell $Metabolism^{†}$

Water in the polar and nonpolar cavities of the protein interleukin-1ß.

Computer-Controlled System for the Study of Oxidase Reactions: Application to the Peroxidase−Oxidase $Oscillator^{†}$

Packaging HIV virion components through dynamic equilibria of a human tRNA synthetase.

Distance-Dependent Diffusion-Controlled Reaction of $^{•}NO$ and $O_{2}^{•−}$ at Chemical Equilibrium with $ONOO^{−}$

Molecular Dynamics Study of the Structures and Dynamics of the Iodine Molecules Confined in $AlPO_{4}-11$ Crystals

Condensation/Evaporation Transition of Water in Spherical Pores in Equilibrium with Saturated Bulk Water

Influence of Sequence on the Self-Assembly of Peptide Nanoribbons on Silicon Substrates

Toward an Understanding of the Salting-Out Effects in Aqueous Ionic Liquid Solutions: Vapor−Liquid Equilibria, Liquid−Liquid Equilibria, Volumetric, Compressibility, and Conductivity Behavior

Origin of the Low-Viscosity of $[emim][(FSO_{2})_{2}N]$ Ionic Liquid and Its Lithium Salt Mixture: Experimental and Theoretical Study of Self-Diffusion Coefficients, Conductivities, and Intermolecular Interactions

Tween 80−Sodium Deoxycholate Mixed Micelles: Structural Characterization and Application in Doxorubicin Delivery

Electrogravimetric Real-Time and in Situ Michaelis−Menten Enzimatic Kinetics: Progress Curve of Acetylcholinesterase Hydrolysis

Photochromism and Photopolymerization Induced Mesophase Transitions in Mixtures of Spiropyran and Mesogenic Diacrylate

Monte Carlo Simulation of Self-Assembly of Symmetric ABC Three-Arm Star Copolymers under Cylindrical Confinement

Competitive Mechanistic Pathways for Green-to-Red Photoconversion in the Fluorescent Protein Kaede: A Computational Study

Heterogeneities in Gelatin Film Formation Using Single-Sided NMR

Weak Epitaxy Growth of Phthalocyanine on 2,5-Bis(4-1,1′:4′,1′′-terphenyl)-thiophene and the Effect of Phase State of Inducing Layer

Polarized Cluster Dynamics at the Paraelectric to Ferroelectric Phase Transition in $BaTiO_{3}$

Relaxation Processes in the Adsorption of Surface Layer Proteins to Lipid Membranes

Pairwise Substitution Effects, Inter- and Intramolecular Hydrogen Bonds in Methoxyphenols and Dimethoxybenzenes. Thermochemistry, Calorimetry, and First-Principles Calculations

Molecular Simulations on the Thermal Stabilization of DNA by Hyperthermophilic Chromatin Protein Sac7d, and Associated Conformational Transitions

Theoretical and Experimental Insights into the Mechanism of the Nucleophilic Addition of Water and Methanol to Dicyanonitrosomethanide

Influence of Structure on the Spectroscopic Properties of the Polymorphs of Piroxicam

Difference in Hydration between Carboxybetaine and Sulfobetaine

Rapid, accurate and simple model to predict NMR chemical shifts for biological molecules.

Molecular Dynamics Investigation of Dipeptide - Transition Metal Salts in Aqueous Solutions

Studies on the Structure and Stability of Cyclic Peptide Based Nanotubes Using Oligomeric Approach: A Computational Chemistry Investigation

Proton Transport Properties in Zwitterion Blends with Brønsted Acids

Experimental and Computational Thermochemical Study of α-Alanine (DL) and β-Alanine

Femtosecond Dynamics of a Porphyrin Derivative Confined by the Human Serum Albumin Protein

Toward an Understanding of the Aqueous Solubility of Amino Acids in the Presence of Salts: A Molecular Dynamics Simulation Study

Thermodynamics of Fatty Acid Degradation

Chain Length Effect on the Binding of Amphiphiles to Serum Albumin and to POPC Bilayers

Specific Features of the Dielectric Continuum Solvation Model with a Position-Dependent Permittivity Function

Theoretical Studies of Salt-Bridge Formation by Amino Acid Side Chains in Low and Medium Polarity Environments

Microstructure and Hydrogen Bonding in Water−Acetonitrile Mixtures

Parametrization and Application of a Coarse Grained Force Field for Benzene/Fullerene Interactions with Lipids

Near-Infrared Luminescence from Visible-Light-Sensitized Hybrid Materials Covalently Linked with Tris(8-hydroxyquinolinate)-lanthanide [Er(III), Nd(III), and Yb(III)] Derivatives

Orientational Order of Colloidal Disk-Shaped Particles under Shear-Flow Conditions: a Rheological−Small-Angle X-ray Scattering Study

Radical Chemistry of 8-Oxo-7,8-dihydro-2′-deoxyadenosine and 8-Oxo-7,8-dihydro-2′-deoxyguanosine: A Pulse Radiolysis Study

Physicochemical Properties and Activity Coefficients at Infinite Dilution for Organic Solutes and Water in the Ionic Liquid 1-Decyl-3-methylimidazolium Tetracyanoborate

Influence of Protein Environment on the Electron Paramagnetic Resonance Properties of Flavoprotein Radicals: A QM/MM Study

On the Use of Excess Entropy Scaling To Describe Single-Molecule and Collective Dynamic Properties of Hydrocarbon Isomer Fluids

Structural Properties of 1-Alkyl-3-methylimidazolium Bis{(trifluoromethyl)sulfonyl}amide Ionic Liquids: X-ray Diffraction Data and Molecular Dynamics Simulations

Determination of Protein Surface Hydration Shell Free Energy of Water Motion: Theoretical Study and Molecular Dynamics Simulation

Sampling the Structure of the Noncanonical Lin-4:Lin-14 microRNA:mRNA Complex by Molecular Dynamics Simulations

Effects of Salt on the Lower Critical Solution Temperature of Poly (N-Isopropylacrylamide)

Professor Robert A. Alberty—A Legacy of Excellence

Brief scientific autobiography of Robert A. Alberty.

Publications of Robert A. Alberty.

Partial Molal Volumes and Compressibilities of Phosphoric Acid and Sodium Phosphates in 0.725 Molal NaCl at 25 $°C^{†}$

Symmetry numbers for rigid, flexible, and fluxional molecules: theory and applications.

Year: 2010 Volume: 114 Issue: 48

Year: 2010 Volume: 114 Issue: 47

Year: 2010 Volume: 114 Issue: 46

Year: 2010 Volume: 114 Issue: 45

Year: 2010 Volume: 114 Issue: 44

Year: 2010 Volume: 114 Issue: 43

Year: 2010 Volume: 114 Issue: 42

Year: 2010 Volume: 114 Issue: 41

Year: 2010 Volume: 114 Issue: 40

Year: 2010 Volume: 114 Issue: 39

Year: 2010 Volume: 114 Issue: 38

Year: 2010 Volume: 114 Issue: 37

Year: 2010 Volume: 114 Issue: 36

Year: 2010 Volume: 114 Issue: 35

Year: 2010 Volume: 114 Issue: 34

Year: 2010 Volume: 114 Issue: 33

Year: 2010 Volume: 114 Issue: 32

Year: 2010 Volume: 114 Issue: 31

Year: 2010 Volume: 114 Issue: 30

Year: 2010 Volume: 114 Issue: 29

Year: 2010 Volume: 114 Issue: 28

Year: 2010 Volume: 114 Issue: 27

Year: 2010 Volume: 114 Issue: 26

Year: 2010 Volume: 114 Issue: 25

Year: 2010 Volume: 114 Issue: 24

Year: 2010 Volume: 114 Issue: 23

Year: 2010 Volume: 114 Issue: 22

Year: 2010 Volume: 114 Issue: 21

Year: 2010 Volume: 114 Issue: 20

Year: 2010 Volume: 114 Issue: 19

Year: 2010 Volume: 114 Issue: 18

Year: 2010 Volume: 114 Issue: 17

Year: 2010 Volume: 114 Issue: 16

Year: 2010 Volume: 114 Issue: 15

Year: 2010 Volume: 114 Issue: 14

Year: 2010 Volume: 114 Issue: 13

Year: 2010 Volume: 114 Issue: 12

Year: 2010 Volume: 114 Issue: 11

Year: 2010 Volume: 114 Issue: 10

Year: 2010 Volume: 114 Issue: 9

Year: 2010 Volume: 114 Issue: 8

Year: 2010 Volume: 114 Issue: 7

Year: 2010 Volume: 114 Issue: 6

Year: 2010 Volume: 114 Issue: 5

Year: 2010 Volume: 114 Issue: 4

Year: 2010 Volume: 114 Issue: 3

Year: 2010 Volume: 114 Issue: 2

Year: 2010 Volume: 114 Issue: 1

Year: 2009 Volume: 113

Year: 2008 Volume: 112

Year: 2007 Volume: 111

Year: 2006 Volume: 110

Year: 2005 Volume: 109

Year: 2004 Volume: 108

Year: 2003 Volume: 107

Year: 2002 Volume: 106

Year: 2001 Volume: 105

Year: 1999 Volume: 103

Year: 1998 Volume: 102

Year: 1997 Volume: 101

Kinetic and spectroscopic evidence of negative cooperativity in the action of lysine 2,3-aminomutase.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Frey, Perry A. Ruzicka, Frank J.
Description	Author Affiliation: Ruzicka FJ ( Department of Biochemistry, University of Wisconsin-Madison, 1710 University Avenue, Madison, Wisconsin 53726, USA.)
Abstract	Lysine 2,3-aminomutase (LAM) catalyzes the interconversion of L-lysine and L-ß-lysine, a component of a number of antibiotics. The reaction requires the cofactors S-adenosyl-L-methionine (SAM), pyridoxal-5'-phosphate (PLP), and a [4Fe-4S] cluster. LAM is a founding member of the radical SAM superfamily of enzymes. LAM is highly specific for L-lysine and will not accept most other amino acids as substrates. L-alanine and L-2-aminobutyrate at 0.2 M react as substrates for LAM at, respectively, 5 × 10(-6) and 8 × 10(-5) times the rate with saturating L-lysine. Saturating ethylamine accelerates the L-alanine reaction 70-fold, and saturating methylamine accelerates the L-2-aminobutyrate reaction 47-fold. The primary amines binding at the active site of LAM with L-alanine or L-2-aminobutyrate simulate L-lysine. The steady-state kinetics of the reaction of L-alanine + ethylamine displays negative cooperativity with respect to L-alanine. The second-order rate constant for production of ß-alanine in the reaction of L-alanine and saturating ethylamine is 0.040 M(-1) s(-1), which is 2 × 10(-5) times the value of k(cat)/K(m) for the reaction of L-lysine. When L-lysine is at a concentration 1/16th of K(m), the lysyl-free radical intermediate is hardly detectable by EPR; however, the addition of L-alanine at high concentration (0.2 M) enhances free radical formation, and the addition of ethylamine further enhances radical formation. These facts complement the kinetic observations and support negative cooperativity in the reaction of L-alanine as a substrate for LAM. Present results and independent evidence support negative cooperativity in the reaction of L-lysine as well.
ISSN	15206106
e-ISSN	15205207
Journal	The Journal of Physical Chemistry B
Issue Number	49
Volume Number	114
Language	English
Publisher	American Chemical Society (United States)
Publisher Date	2010-12-16
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Intramolecular Transferases Chemistry Catalytic Domain Kinetics Molecular Structure Beta-Alanine Metabolism Research Support, N.I.H., Extramural Physical chemistry
Content Type	Text
Resource Type	Article
Subject	Surfaces, Coatings and Films Materials Chemistry Medicine Physical and Theoretical Chemistry

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in