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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Raff, R. A. Gross, K. W. Greenhouse, G. Gross, P. R. |
| Abstract | Studies employing colchicine binding, precipitation with vinblastine sulfate, and acrylamide gel electrophoresis confirm earlier proposals that Arbacia punctulata and Lytechinus pictus eggs and embryos contain a store of microtubule proteins. Treatment of 150,000 g supernatants from sea urchin homogenates with vinblastine sulfate precipitates about 5% of the total soluble protein, and 75% of the colchicine-binding activity. Electrophoretic examination of the precipitate reveals two very prominent bands. These have migration rates identical to those of the A and B microtubule proteins of cilia. These proteins can be made radioactive at the 16 cell stage and at hatching by pulse labeling with tritiated amino acids. By labeling for 1 hr with leucine-(3)H in early cleavage, then culturing embryos in the presence of unlabeled leucine, removal of newly synthesized microtubule proteins from the soluble pool can be demonstrated. Incorporation of labeled amino acids into microtubule proteins is not affected by culturing embryos continuously in 20 microg/ml of actinomycin D. Microtubule proteins appear, therefore, to be synthesized on 'maternal' messenger RNA. This provides the first protein encoded by stored or 'masked' mRNA in sea urchin embryos to be identified. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 2 |
| Volume Number | 50 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1971-08-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Microtubules Metabolism Protein Biosynthesis Amino Acids Animals Cell Division Colchicine Dactinomycin Pharmacology Electrophoresis, Disc Leucine Ovum Protein Binding Proteins Antagonists & Inhibitors RNA, Messenger Sea Urchins Embryology Spermatozoa Time Factors Tritium Vinblastine Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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