NDLI: Isolation of germ cell Golgi apparatus from seminiferous tubules of rat testes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Cunningham, W. P. Mollenhauer, H. H. Nyquist, S. E.
Abstract	Intact Golgi apparatus have been isolated with good purity from rat testis by a simplified sucrose gradient technique. The procedure is inherently selective in that most of the Golgi apparatus in the isolate are from germ cells in late spermatocyte or early spermatid development. No Sertoli cell Golgi apparatus are present in the fraction. Biochemical analyses showed that the enzyme N-acetylglucosamine galactosyltransferase is enhanced in the band containing Golgi apparatus. Because they are easy to isolate, and because they are involved with the formation of a specific internal cellular component (the acrosome), these Golgi apparatus will be useful objects for comparison with other kinds of Golgi apparatus and for other studies leading to a better understanding of the basic functioning of Golgi apparatus.
ISSN	00219525
e-ISSN	15408140
Journal	The Journal of Cell Biology
Issue Number	1
Volume Number	51
Language	English
Publisher	Rockefeller University Press (United States)
Publisher Date	1971-10-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Golgi Apparatus Spermatozoa Testis Cytology Animals Cell Fractionation Culture Media Endoplasmic Reticulum Enzymology Microscopy, Electron Rats, Inbred Strains Spermatogenesis Transferases Ultracentrifugation Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine

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Isolation of germ cell Golgi apparatus from seminiferous tubules of rat testes.