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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Tilney, L. G. Mooseker, M. S. Hatano, S. Ishikawa, H. |
| Abstract | When Asterias or Thyone sperm come in contact with egg jelly, a long process which in Thyone measures up to 90 µm in length is formed from the acrosomal region. This process can be generated in less than 30 s. Within this process is a bundle of microfilaments. Water extracts prepared from acetone powders of Asterias sperm contain a protein which binds rabbit skeletal muscle myosin forming a complex whose viscosity is reduced by ATP. Within this extract is a protein with the same molecular weight as muscle actin. It can be purified either by collecting the pellet produced after the addition of $Mg^{++}$ or by reextracting an acetone powder of actomyosin prepared by the addition of highly purified muscle myosin to the extract. The sperm actin can be polymerized and by electron microscopy the polymer is indistinguishable from muscle F-actin. The sperm actin was shown to be localized in the microfilaments in the acrosomal processes by: (a) heavy meromyosin binding in situ, (b) sodium dodecyl sulfate (SDS) gel electrophoresis of the isolated acrosomal processes and a comparison to gels of flagella which contain no band corresponding to the molecular weight of actin, and (c) SDS gel electrophoresis of the extract from isolated acrosomal caps. Since the precursor for the microfilaments in the unreacted sperm appears amorphous, we suspected that the force for the generation of the acrosomal process is brought about by the polymerization of the sperm actin. This supposition was confirmed, for when unreacted sperm were lysed with the detergent Triton X-100 and the state of the actin in the sperm extract was analyzed by centrifugation, we determined that at least 80% of the actin in the unreacted sperm was in the monomeric state. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 1 |
| Volume Number | 59 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1973-10-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Actins Physiology Echinodermata Spermatozoa Cytology Starfish Isolation & Purification Animals Cell Fractionation Chemical Phenomena Chemistry Electrophoresis, Polyacrylamide Gel Inclusion Bodies Microscopy, Electron Molecular Weight Myosin Subfragments Metabolism Myosins Polymers Sodium Dodecyl Sulfate Viscosity Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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