NDLI: A ZONAL ROTOR METHOD FOR THE PREPARATION OF MICROPEROXISOMES FROM EPITHELIAL CELLS OF GUINEA PIG SMALL INTESTINE

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Connock, M. J. Kirk, P. R. Sturdee, A. P.
Abstract	A method is described for the preparation of catalase particles from homogenates made from suspensions of epithelial cells of the small intestine of the guinea pig. Electron microscope examination of the preparations revealed the presence of small diaminobenzidine-positive particles measuring 0.1–0.3 nm in diameter and resembling the microperoxisomes observed by Novikoff and Novikoff (1972. J. Cell Biol. 53:532.). Analytical data upon which the method is based are presented. The technique consisted of a rate zonal separation of microperoxisomes from large particles followed by an isopycnic separation from less dense organelles. Application of the method yielded microperoxisomes purified between 20- and 30-fold.
ISSN	00219525
e-ISSN	15408140
Journal	The Journal of Cell Biology
Issue Number	1
Volume Number	61
Language	English
Publisher	Rockefeller University Press (United States)
Publisher Date	1974-04-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cell Fractionation Intestine, Small Cytology Organoids Alkaline Phosphatase Animals Catalase Centrifugation, Density Gradient Epithelial Cells Esterases Guinea Pigs Lysosomes Enzymology Microbodies Microscopy, Electron Microsomes Mitochondria Proteins Staining And Labeling Subcellular Fractions Succinate Dehydrogenase Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine

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A ZONAL ROTOR METHOD FOR THE PREPARATION OF MICROPEROXISOMES FROM EPITHELIAL CELLS OF GUINEA PIG SMALL INTESTINE