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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Griffiths, G. Olsnes, S. Sandvig, K. Van Deurs, B. Petersen, O. W. Simons, K. |
| Description | Author Affiliation: van Deurs B ( Department of Anatomy, Panum Institute, University of Copenhagen, Denmark.) |
| Abstract | We have used a protocol for internalization of ricin, a ligand binding to plasma membrane glycoproteins and glycolipids with terminal galactosyl residues, and infection with the vesicular stomatitis virus ts 045 mutant in BHK-21 cells to determine whether internalized plasma membrane molecules tagged by ricin reach distinct compartments of the biosynthetic-exocytic pathway. At 39.5 degrees C newly synthesized G protein of ts 045 was largely prevented from leaving the endoplasmic reticulum. At the same temperature ricin was endocytosed and reached, in addition to endosomes and lysosomes, elements of the Golgi complex. When the temperature was lowered to 19.5 degrees C, no more ricin was delivered to the Golgi complex, but now G protein accumulated in the Golgi stacks and the trans-Golgi network (TGN). Double-labeling immunogold cytochemistry on ultracryosections was used to detect G protein and ricin simultaneously. These data, combined with stereological and biochemical methods, showed that approximately 5% of the total amount of ricin within the cells, corresponding to 6-8 X 10(4) molecules per cell, colocalized with G protein in the Golgi complex after 60 min at 39.5 degrees C. Of this amount approximately 70- 80% was present in the TGN. Since most of the ricin molecules remain bound to their binding sites at the low pH prevailing in compartments of the endocytic pathway, the results indicate that a fraction of the internalized plasma membrane molecules with terminal galactose are not recycled directly from endosomes or delivered to lysosomes, but are routed to the Golgi complex. Also, the results presented here, in combination with other recent studies on ricin internalization, suggest that translocation of the toxic ricin A-chain to the cytosol occurs in the TGN. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 2 |
| Volume Number | 106 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1988-02-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Endocytosis Golgi Apparatus Metabolism Ricin Viral Envelope Proteins Animals Biological Transport Cell Compartmentation Cell Line Cricetinae Endosomes Fluorescent Antibody Technique Immunohistochemistry Intracellular Membranes Membrane Glycoproteins Vesicular Stomatitis Indiana Virus Viral Matrix Proteins Research Support, Non-U.S. Gov't Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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