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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Forscher, P. Smith, S. J. |
| Description | Author Affiliation: Forscher P ( Howard Hughes Medical Institute Research Laboratories, Yale University School of Medicine, Section of Molecular Neurobiology, New Haven, Connecticut 06510.) |
| Abstract | Actions of cytochalasin B (CB) on cytoskeletons and motility of growth cones from cultured Aplysia neurons were studied using a rapid flow perfusion chamber and digital video light microscopy. Living growth cones were observed using differential interference contrast optics and were also fixed at various time points to assay actin filament (F- actin) and microtubule distributions. Treatment with CB reversibly blocked motility and eliminated most of the phalloidin-stainable F- actin from the leading lamella. The loss of F-actin was nearly complete within 2-3 min of CB application and was largely reversed within 5-6 min of CB removal. The loss and recovery of F-actin were found to occur with a very distinctive spatial organization. Within 20-30 s of CB application, F-actin networks receded from the entire peripheral margin of the lamella forming a band devoid of F-actin. This band widened as F- actin receded at rates of 3-6 microns/min. Upon removal of CB, F-actin began to reappear within 20-30 s. The initial reappearance of F-actin took two forms: a coarse isotropic matrix of F-actin bundles throughout the lamella, and a denser matrix along the peripheral margin. The denser peripheral matrix then expanded in width, extending centrally to replace the coarse matrix at rates again between 3-6 microns/min. These results suggest that actin normally polymerizes at the leading edge and then flows rearward at a rate between 3-6 microns/min. CB treatment was also observed to alter the distribution of microtubules, assayed by antitubulin antibody staining. Normally, microtubules are restricted to the neurite shaft and a central growth cone domain. Within approximately 5 min after CB application, however, microtubules began extending into the lamellar region, often reaching the peripheral margin. Upon removal of CB, the microtubules were restored to their former central localization. The timing of these microtubule redistributions is consistent with their being secondary to effects of CB on lamellar F-actin. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 4 |
| Volume Number | 107 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1988-10-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Actin Cytoskeleton Drug Effects Cytochalasins Pharmacology Cytoskeleton Microtubules Neurons Physiology Ultrastructure Actins Animals Aplysia Cell Movement Cells, Cultured Image Processing, Computer-Assisted In Vitro Techniques Polymers Time Factors Video Recording Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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