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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Huttner, W. B. Rosa, P. Weiss, U. Stelzer, E. H. Niehrs, C. Pepperkok, R. Ansorge, W. |
| Description | Author Affiliation: Rosa P ( Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.) |
| Abstract | We have investigated the sorting and packaging of secretory proteins into secretory granules by an immunological approach. An mAb against secretogranin I (chromogranin B), a secretory protein costored with various peptide hormones and neuropeptides in secretory granules of many endocrine cells and neurons, was expressed by microinjection of its mRNA into the secretogranin I-producing cell line PC12. An mAb against the G protein of vesicular stomatitis virus--i.e., against an antigen not present in PC12 cells--was expressed as a control. The intracellular localization and the secretion of the antibodies was studied by double-labeling immunofluorescence using the conventional and the confocal microscope, as well as by pulse-chase experiments. The secretogranin I antibody, like the control antibody, was transported along the secretory pathway to the Golgi complex. However, in contrast to the control antibody, which was secreted via the constitutive pathway, the secretogranin I antibody formed an immunocomplex with secretogranin I, was packaged into secretory granules, and was released by regulated exocytosis. Our results show that a constitutive secretory protein, unaltered by genetic engineering, can be diverted to the regulated pathway of secretion by its protein-protein interaction with a regulated secretory protein. The data also provide the basis for immunologically studying the role of luminally exposed protein domains in the biogenesis and function of regulated secretory vesicles. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 1 |
| Volume Number | 109 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1989-07-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Antigen-Antibody Complex Metabolism Cytoplasmic Granules Proteins Immunology Ammonium Chloride Pharmacology Animals Antibodies, Monoclonal Secretion Blotting, Western Cell Compartmentation Drug Effects Cell Line Chromogranin B Chromogranins Fluorescent Antibody Technique Microinjections Pheochromocytoma Precipitin Tests Spider Venoms Research Support, Non-U.S. Gov't Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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