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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Stirzaker, S. C. Both, G. W. Poncet, D. |
| Description | Author Affiliation: Stirzaker SC ( Commonwealth Scientific and Industrial Research Organization Division of Biomolecular Engineering, North Ryde, NSW, Australia.) |
| Abstract | Glycosylation and translocation of the simian rotavirus protein VP7, a resident ER protein, does not occur co-translationally in vivo. In pulse-chase experiments in COS cells, nonglycosylated VP7 was still detectable after a 25-min chase period, although the single glycosylation site was only 18 residues beyond the signal peptide cleavage site. After labeling, glycosylated and nonglycosylated VP7 was recovered in microsomes but the latter was sensitive to trypsin (i.e., the nascent protein became membrane associated) but most of it entered the ER posttranslationally because of a rate-limiting step early in translocation. In contrast with the simian protein, bovine VP7 was glycosylated and translocated rapidly. Thus, delayed translocation per se was not required for retention of VP7 in the ER. By constructing hybrid proteins, it was further shown that the signal peptide together with residues 64-111 of the simian protein caused delayed translocation. The same sequences were also necessary and sufficient for retention of simian VP7 in the ER. The data are consistent with the idea that certain proteins are inserted into the ER membrane in a loop configuration. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 4 |
| Volume Number | 111 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1990-10-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Capsid Proteins Capsid Chemistry Endoplasmic Reticulum Metabolism Amino Acid Sequence Animals Antigens, Viral Biological Transport Physiology Glycosylation Kinetics Molecular Sequence Data Protein Sorting Signals Rotavirus Transfection Research Support, Non-U.S. Gov't Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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