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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Toma, J. G. Miller, F. D. Mathew, T. C. |
| Description | Author Affiliation: Miller FD ( Department of Anatomy and Cell Biology, University of Alberta, Edmonton, Canada.) |
| Abstract | Nerve growth factor (NGF) is a target-derived neurotrophic protein that promotes the survival and growth of developing sympathetic and sensory neurons. We have examined NGF receptor gene expression in these neurons after NGF administration. Northern blot and in situ hybridization analyses demonstrated that NGF given systemically to neonatal rats increased levels of NGF receptor mRNA in sympathetic neurons within the superior cervical ganglion. This increase was accompanied by a differential regulation of genes associated with neurotransmitter phenotype; tyrosine hydroxylase mRNA was increased, but neuropeptide Y mRNA was not. NGF receptor mRNA levels were also increased in L4-L5 dorsal root ganglia, although this mRNA was not expressed uniformly in sensory neurons of control or NGF-treated animals. Levels of T alpha 1 alpha-tubulin mRNA, a marker of neuronal growth, also increased. In contrast to developing neurons, systemic NGF did not increase NGF receptor mRNA in nonneuronal cells of the sciatic nerve. To determine if NGF regulated NGF receptor gene expression at the transcriptional level, we examined PC12 cells. NGF treatment for 6 h increased NGF receptor mRNA fourfold; this increase was inhibited by cycloheximide. Nuclear run-off transcription assays demonstrated that the increase in steady-state NGF receptor mRNA levels was mediated at the transcriptional level. In contrast, although NGF treatment increased steady-state tyrosine hydroxylase mRNA levels, this effect was not blocked by cycloheximide, and was not due to increased transcription. These data raise the possibility that transcriptional regulation of NGF receptor gene expression by target-derived NGF could be a molecular mechanism for potentiating NGF's effects on neurons during developmental periods of neuronal competition and cell death. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 2 |
| Volume Number | 112 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1991-01-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Adrenergic Fibers Metabolism Gene Expression Regulation Nerve Growth Factors Pharmacology Neurons, Afferent Receptors, Cell Surface Genetics Animals Animals, Newborn Blotting, Northern Cycloheximide Ganglia, Spinal Cytology Ganglia, Sympathetic Genes RNA, Messenger Rats, Inbred Strains Receptors, Nerve Growth Factor Sciatic Nerve Transcription, Genetic Tubulin Tumor Cells, Cultured Tyrosine 3-Monooxygenase Research Support, Non-U.S. Gov't Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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