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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bretscher, A. Liu, H. |
| Description | Author Affiliation: Liu H ( Section of Biochemistry, Cornell University, Ithaca, New York 14853.) |
| Abstract | Disruption of the yeast tropomyosin gene TPM1 results in the apparent loss of actin cables from the cytoskeleton (Liu, H., and A. Bretscher. 1989. Cell. 57:233-242). Here we show that TPM1 disrupted cells grow slowly, show heterogeneity in cell size, have delocalized deposition of chitin, and mate poorly because of defects in both shmooing and cell fusion. The transit time of alpha-factor induced a-agglutinin secretion to the cell surface is longer than in isogenic wild-type strains, and some of the protein is mislocalized. Many of the TPM1-deleted cells contain abundant vesicles, similar in morphology to late secretory vesicles, but without an abnormal accumulation of intermediates in the delivery of either carboxypeptidase Y to the vacuole or invertase to the cell surface. Combinations of the TPM1 disruption with sec13 or sec18 mutations, which affect early steps in the secretory pathway, block vesicle accumulation, while combinations with sec1, sec4 or sec6 mutations, which affect a late step in the secretory pathway, have no effect on the vesicle accumulation. The phenotype of the TPM1 disrupted cells is very similar to that of a conditional mutation in the MYO2 gene, which encodes a myosin-like protein (Johnston, G. C., J. A. Prendergast, and R. A. Singer. 1991. J. Cell Biol. 113:539-551). The myo2-66 conditional mutation shows synthetic lethality with the TPM1 disruption, indicating that the MYO2 and TPM1 gene products may be involved in the same, or parallel function. We conclude that tropomyosin, and by inference actin cables, may facilitate directed vesicular transport of components to the correct location on the cell surface. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 2 |
| Volume Number | 118 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1992-07-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Chromosome Deletion Cytoplasmic Granules Ultrastructure Genes, Fungal Glycoside Hydrolases Metabolism Saccharomyces Cerevisiae Tropomyosin Genetics Chitin Crosses, Genetic Physiology Diploidy Genotype Glycosylation Kinetics Microscopy, Electron Peptides Pheromones Cytology Beta-Fructofuranosidase Research Support, U.S. Gov't, P.H.S. Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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