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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kornbluth, S. Dasso, M. Newport, J. |
| Description | Author Affiliation: Kornbluth S ( Department of Biology, University of California, San Diego, La Jolla 92193.) |
| Abstract | TC4, a ras-like G protein, has been implicated in the feedback pathway linking the onset of mitosis to the completion of DNA replication. In this report we find distinct roles for TC4 in both nuclear assembly and cell cycle progression. Mutant and wild-type forms of TC4 were added to Xenopus egg extracts capable of assembling nuclei around chromatin templates in vitro. We found that a mutant TC4 protein defective in GTP binding (GDP-bound form) suppressed nuclear growth and prevented DNA replication. Nuclear transport under these conditions approximated normal levels. In a separate set of experiments using a cell-free extract of Xenopus eggs that cycles between S and M phases, the GDP- bound form of TC4 had dramatic effects, blocking entry into mitosis even in the complete absence of nuclei. The effect of this mutant TC4 protein on cell cycle progression is mediated by phosphorylation of p34cdc2 on tyrosine and threonine residues, negatively regulating cdc2 kinase activity. Therefore, we provide direct biochemical evidence for a role of TC4 in both maintaining nuclear structure and in the signaling pathways that regulate entry into mitosis. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 4 |
| Volume Number | 125 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1994-05-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Cell Cycle Physiology Cell Nucleus Nuclear Proteins Amino Acid Sequence Animals Biological Transport CDC2 Protein Kinase Metabolism Ultrastructure DNA DNA Replication Guanosine Triphosphate Mitosis Molecular Sequence Data Mutation Genetics Signal Transduction Xenopus Ran GTP-Binding Protein Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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