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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Beebe, L. A. Yan, J. P. Colon, M. E. Melançon, P. |
| Description | Author Affiliation: Yan JP ( Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309-0215.) |
| Abstract | 22 CHOBFY (BFY) cell lines were isolated at a frequency 2-30 x 10(-7) from mutagenized populations on the basis of their ability to grow in the presence of 1 microgram/ml brefeldin A (BFA). Four of the five mutant lines tested are genetically stable and none of the mutant lines characterized degrade this drug. Immunofluorescence studies reveal that whereas early endosomes and the Golgi complex have nearly identical BFA sensitivities in the parent CHO line, the relative sensitivities of these two organelles were dramatically altered in all six mutant lines tested. Four cell lines maintain normal Golgi appearance at a BFA concentration as high as 10 micrograms/ml. Mutant lines show wide variation in the level of resistance to growth inhibition by BFA, but none of the mutant lines characterized grow above 2 micrograms/ml BFA. This specific growth inhibition is observed under conditions where Golgi morphology and function remain unaffected, suggesting that some factor(s) unrelated to Golgi function remains sensitive to BFA in BFY mutant lines. These observations provide strong evidence for the presence of multiple, organelle-specific targets for BFA. Cell-free measurements with membrane extracts establish that resistance to BFA in BFY-1 cells involves a membrane-associated factor distinct from ARFs and coatomers. This collection of mutant lines may prove valuable for the identification of intracellular target(s) for BFA and/or of effectors that interact upstream or downstream with these targets, thereby uncovering the cascade which regulates assembly of organelle- specific coats. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 1 |
| Volume Number | 126 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1994-07-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | CHO Cells Physiology Cell Compartmentation Cyclopentanes Pharmacology Membrane Glycoproteins Mutation Organelles Drug Effects ADP-Ribosylation Factors Animals Brefeldin A Cell Division Coatomer Protein Cricetinae Metabolism Dose-Response Relationship, Drug Drug Resistance Genetics GTP-Binding Proteins Golgi Apparatus Ultrastructure Membrane Proteins Microtubule-Associated Proteins Models, Biological Phenotype Selection, Genetic Viral Envelope Proteins Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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