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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Sweet, D. J. Gerace, L. |
| Description | Author Affiliation: Sweet DJ ( Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037, USA.) |
| Abstract | Signal-dependent transport of proteins into the nucleus is a multi-step process mediated by nuclear pore complexes and cytosolic transport factors. One of the cytosolic factors, Ran, is the only GTPase that has a characterized role in the nuclear import pathway. We have used a mutant form of Ran with altered nucleotide binding specificity to investigate whether any other GTPases are involved in nuclear protein import. D125N Ran (XTP-Ran) binds specifically to xanthosine triphosphate (XTP) and has a greatly reduced affinity for GTP, so it is no longer sensitive to inhibition by nonhydrolyzable analogues of GTP such as guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S). using in vitro transport assays, we have found that nuclear import supported by XTP-Ran is nevertheless inhibited by the addition of non-hydrolyzable GTP analogues. This in conjunction with the properties of the inhibitory effect indicates that at least one additional GTPase is involved in the import process. Initial characterization suggests that the inhibited GTPase plays a direct role in protein import and could be a component of the nuclear pore complex. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 5 |
| Volume Number | 133 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1996-06-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | GTP Phosphohydrolases Metabolism Nuclear Proteins Biological Transport, Active Drug Effects Cytosol Guanosine Triphosphate Analogs & Derivatives Pharmacology HeLa Cells Nuclear Envelope Genetics Nucleotides Point Mutation Ribonucleotides Ran GTP-Binding Protein Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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