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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Radhakrishna, H. Klausner, R. D. Donaldson, J. G. |
| Description | Author Affiliation: Radhakrishna H ( Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.) |
| Abstract | To study the effector function of the ADP- ribosylation factor (ARF) 6 GTP-binding protein, we transfected HeLa cells with wild-type, epitope- tagged ARF6. Previously shown to indirectly activate the ARF1 GTPase, aluminum fluoride (AIF) treatment of ARF6-transfected cells resulted in a redistribution of both ARF6 and actin to discrete sites on the plasma membrane, which became increasingly protrusive over time. The effects of AIF were reversible, specific to cells transfected with wild-type ARF6, and resembled the cellular protrusions observed in cells expressing the GTPase defective mutant of ARF6. Importantly, the protrusions observed in cells transfected with ARF6 were distinct from the enhanced stress fibers and membrane ruffles observed in cells transfected with RhoA and Rac1, respectively. In cells forming protrusions, there was an apparent stimulation of macropinocytosis and membrane recycling within the protrusive structures. In contrast, no block in transferrin uptake or alteration of the distribution of clathrin AP-2 complexes was detected in these cells. The AIF-induced, ARF6- dependent formation of protrusive structures was blocked by cytochalasin D and inhibitors of the lipoxygenase pathway. These observations support a novel role for the ARF6 GTPase in modeling the plasma membrane and underlying cytoskeleton. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 4 |
| Volume Number | 134 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1996-08-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Aluminum Compounds Pharmacology Cell Membrane Drug Effects Fluorides GTP Phosphohydrolases Metabolism GTP-Binding Proteins Membrane Proteins ADP-Ribosylation Factor 1 ADP-Ribosylation Factors Actins Arachidonic Acid Cell Adhesion Molecules Clathrin Cortactin Cytochalasin D Focal Adhesion Kinase 1 Focal Adhesion Protein-Tyrosine Kinases Biosynthesis Genetics Gelsolin HeLa Cells Lipoxygenase Inhibitors Masoprocol Microfilament Proteins Mutation Phospholipases A Antagonists & Inhibitors Pinocytosis Protein-Tyrosine Kinases Pseudopodia Transfection Transferrin Rac GTP-Binding Proteins RhoA GTP-Binding Protein Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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