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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Saunders, W. Lengyel, V. Deng, C. Hornack, D. |
| Description | Author Affiliation: Saunders W ( Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260, USA. wsaund@vms.cis.pitt.edu) |
| Abstract | The Saccharomyces cerevisiae kinesin-related motor Kar3p, though known to be required for karyogamy, plays a poorly defined, nonessential role during vegetative growth. We have found evidence suggesting that Kar3p functions to limit the number and length of cytoplasmic microtubules in a cell cycle–specific manner. Deletion of KAR3 leads to a dramatic increase in cytoplasmic microtubules, a phenotype which is most pronounced from START through the onset of anaphase but less so during late anaphase in synchronized cultures. We have immunolocalized HA-tagged Kar3p to the spindle pole body region, and fittingly, Kar3p was not detected by late anaphase. A microtubule depolymerizing activity may be the major vegetative role for Kar3p. Addition of the microtubule polymerization inhibitors nocodazol or benomyl to the medium or deletion of the nonessential α-tubulin TUB3 gene can mostly correct the abnormal microtubule arrays and other growth defects of kar3 mutants, suggesting that these phenotypes result from excessive microtubule polymerization. Microtubule depolymerization may also be the mechanism by which Kar3p acts in opposition to the anaphase B motors Cin8p and Kip1p. A preanaphase spindle collapse phenotype of cin8 kip1 mutants, previously shown to involve Kar3p, is markedly delayed when microtubule depolymerization is inhibited by the tub2-150 mutation. These results suggest that the Kar3p motor may act to regulate the length and number of microtubules in the preanaphase spindle. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 2 |
| Volume Number | 137 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1997-04-21 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Anaphase Physiology Fungal Proteins Microtubule-Associated Proteins Microtubules Metabolism Saccharomyces Cerevisiae Proteins Saccharomyces Cerevisiae Cytology Spindle Apparatus Chemistry Amino Acid Sequence Benomyl Pharmacology Genetics Kinesin Drug Effects Molecular Motor Proteins Molecular Sequence Data Mutation Nocodazole Recombinant Fusion Proteins Research Support, Non-U.S. Gov't Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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