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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Trimbur, G. M. Fath, K. R. Burgess, D. R. |
| Description | Author Affiliation: Fath KR ( Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA.) |
| Abstract | Cytoplasmic dynein is a microtubule minus-end–directed motor that is thought to power the transport of vesicles from the TGN to the apical cortex in polarized epithelial cells. Trans-Golgi enriched membranes, which were isolated from primary polarized intestinal epithelial cells, contain both the actin-based motor myosin-I and dynein, whereas isolated Golgi stacks lack dynein but contain myosin-I (Fath, K.R., G.M. Trimbur, and D.R. Burgess. 1994. J. Cell Biol. 126:661–675). We show now that Golgi stacks in vitro bind dynein supplied from cytosol in the absence of ATP, and bud small membranes when incubated with cytosol and ATP. Cytosolic dynein binds to regions of stacks that are destined to bud because dynein is present in budded membranes, but absent from stacks after budding. Budded membranes move exclusively towards microtubule minus-ends in in vitro motility assays. Extraction studies suggest that dynein binds to a Golgi peripheral membrane protein(s) that resists extraction by ice-cold Triton X-100. In the presence of cytosol, these membrane ghosts can move towards the minus-ends of microtubules. Detergent-extracted Golgi stacks and TGN-containing membranes are closely associated with an amorphous matrix composed in part of spectrin and ankyrin. Although spectrin has been proposed to help link dynein to organellar membranes, we found that functional dynein may bind to extracted membranes independently of spectrin and ankyrin. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 5 |
| Volume Number | 139 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1997-12-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Cell Polarity Physiology Dyneins Metabolism Golgi Apparatus Spectrin Animals Ankyrins Isolation & Purification Cell-Free System Coatomer Protein Cytosol Epithelial Cells Drug Effects Intestinal Mucosa Microtubule-Associated Proteins Microtubules Movement Myosins Octoxynol Pharmacology Protein Binding Subcellular Fractions Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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