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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | White, J. Röttger, S. Suganuma, T. Nilsson, T. Storrie, B. Stelzer, E. H. |
| Description | Author Affiliation: Storrie B ( Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061-0308, USA. storrie@vt.edu) |
| Abstract | During microtubule depolymerization, the central, juxtanuclear Golgi apparatus scatters to multiple peripheral sites. We have tested here whether such scattering is due to a fragmentation process and subsequent outward tracking of Golgi units or if peripheral Golgi elements reform through a novel recycling pathway. To mark the Golgi in HeLa cells, we stably expressed the Golgi stack enzyme N-acetylgalactosaminyltransferase-2 (GalNAc-T2) fused to the green fluorescent protein (GFP) or to an 11–amino acid epitope, VSV-G (VSV), and the trans/TGN enzyme β1,4-galactosyltransferase (GalT) fused to GFP. After nocodazole addition, time-lapse microscopy of GalNAc-T2–GFP and GalT–GFP revealed that scattered Golgi elements appeared abruptly and that no Golgi fragments tracked outward from the compact, juxtanuclear Golgi complex. Once formed, the scattered structures were relatively stable in fluorescence intensity for tens of minutes. During the entire process of dispersal, immunogold labeling for GalNAc-T2–VSV and GalT showed that these were continuously concentrated over stacked Golgi cisternae and tubulovesicular Golgi structures similar to untreated cells, suggesting that polarized Golgi stacks reform rapidly at scattered sites. In fluorescence recovery after photobleaching over a narrow (FRAP) or wide area (FRAP-W) experiments, peripheral Golgi stacks continuously exchanged resident proteins with each other through what appeared to be an ER intermediate. That Golgi enzymes cycle through the ER was confirmed by microinjecting the dominant-negative mutant of Sar1 $(Sar1p^{dn})$ blocking ER export. $Sar1p^{dn}$ was either microinjected into untreated or nocodazole-treated cells in the presence of protein synthesis inhibitors. In both cases, this caused a gradual accumulation of GalNAc-T2–VSV in the ER. Few to no peripheral Golgi elements were seen in the nocodazole-treated cells microinjected with $Sar1p^{dn}.$ In conclusion, we have shown that Golgi-resident glycosylation enzymes recycle through the ER and that this novel pathway is the likely explanation for the nocodazole-induced Golgi scattering observed in interphase cells. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 6 |
| Volume Number | 143 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1998-12-14 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Endoplasmic Reticulum Enzymology Galactosyltransferases Metabolism Glycosyltransferases Golgi Apparatus Membrane Glycoproteins Monomeric GTP-Binding Proteins N-Acetylgalactosaminyltransferases Saccharomyces Cerevisiae Proteins Ultrastructure GTP-Binding Proteins Genetics Drug Effects Green Fluorescent Proteins HeLa Cells Kinetics Luminescent Proteins Microinjections Microscopy, Immunoelectron Microtubules Nocodazole Pharmacology Recombinant Fusion Proteins Transfection Vesicular Transport Proteins Vesicular Stomatitis Indiana Virus Viral Envelope Proteins Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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