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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Matsuoka, K. Raikhel, N. V. Dombrowski, J. E. Ahmed, S. U. Kovaleva, V. Rojo, E. Venkataraman, S. |
| Description | Author Affiliation: Ahmed SU ( Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824, USA.) |
| Abstract | Many soluble plant vacuolar proteins are sorted away from secreted proteins into small vesicles at the trans-Golgi network by transmembrane cargo receptors. Cleavable vacuolar sorting signals include the $NH_{2}-terminal$ propeptide (NTPP) present in sweet potato sporamin (Spo) and the COOH-terminal propeptide (CTPP) present in barley lectin (BL). These two proteins have been found to be transported by different mechanisms to the vacuole. We examined the ability of the vacuolar cargo receptor AtELP to interact with the sorting signals of heterologous and endogenous plant vacuolar proteins in mediating vacuolar transport in Arabidopsis thaliana. AtELP extracted from microsomes was found to interact with the NTPPs of barley aleurain and Spo, but not with the CTPPs of BL or tobacco chitinase, in a pH-dependent and sequence-specific manner. In addition, EM studies revealed the colocalization of AtELP with NTPP-Spo at the Golgi apparatus, but not with BL-CTPP in roots of transgenic Arabidopsis plants. Further, we found that AtELP interacts in a similar manner with the NTPP of the endogenous vacuolar protein AtALEU (Arabidopsis thaliana Aleu), a protein highly homologous to barley aleurain. We hypothesize that AtELP functions as a vacuolar sorting receptor involved in the targeting of NTPP-, but not CTPP-containing proteins in Arabidopsis. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 7 |
| Volume Number | 149 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 2000-06-26 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Arabidopsis Proteins Arabidopsis Metabolism Peptide Fragments Plant Proteins Protein Structure, Tertiary Vacuoles Chemistry Ultrastructure Binding Sites Physiology Biological Transport Cell Compartmentation Cysteine Endopeptidases Golgi Apparatus Hordeum Hydrogen-Ion Concentration Plant Roots Plants, Genetically Modified Protein Precursors Sequence Analysis, Protein Signal Transduction Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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