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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Welch, M. D. Portnoy, D. A. Skoble, J. |
| Description | Author Affiliation: Skoble J ( Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720-3200, USA.) |
| Abstract | The Listeria monocytogenes ActA protein induces actin-based motility by enhancing the actin nucleating activity of the host Arp2/3 complex. Using systematic truncation analysis, we identified a 136-residue $NH_{2}-terminal$ fragment that was fully active in stimulating nucleation in vitro. Further deletion analysis demonstrated that this fragment contains three regions, which are important for nucleation and share functional and/or limited sequence similarity with host WASP family proteins: an acidic stretch, an actin monomer–binding region, and a cofilin homology sequence. To determine the contribution of each region to actin-based motility, we compared the biochemical activities of ActA derivatives with the phenotypes of corresponding mutant bacteria in cells. The acidic stretch functions to increase the efficiency of actin nucleation, the rate and frequency of motility, and the effectiveness of cell–cell spread. The monomer-binding region is required for actin nucleation in vitro, but not for actin polymerization or motility in infected cells, suggesting that redundant mechanisms may exist to recruit monomer in host cytosol. The cofilin homology sequence is critical for stimulating actin nucleation with the Arp2/3 complex in vitro, and is essential for actin polymerization and motility in cells. These data demonstrate that each region contributes to actin-based motility, and that the cofilin homology sequence plays a principal role in activation of the Arp2/3 complex, and is an essential determinant of L. monocytogenes pathogenesis. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 3 |
| Volume Number | 150 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 2000-08-07 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Actins Metabolism Bacterial Proteins Cytoskeletal Proteins Listeria Monocytogenes Pathogenicity Membrane Proteins Actin-Related Protein 2 Actin-Related Protein 3 Amino Acid Sequence Animals Genetics Binding Sites HeLa Cells Molecular Sequence Data Movement Mutation Peptide Fragments Protein Conformation Proteins Wiskott-Aldrich Syndrome Protein Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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