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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bezanilla, M. Pollard, T. D. Lee, W. L. |
| Description | Author Affiliation: Lee WL ( Graduate Program in Biochemistry, Cellular and Molecular Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.) |
| Abstract | Fission yeast $myo1^{+}$ encodes a myosin-I with all three tail homology domains (TH1, 2, 3) found in typical long-tailed myosin-Is. Myo1p tail also contains a COOH-terminal acidic region similar to the A-domain of WASp/Scar proteins and other fungal myosin-Is. Our analysis shows that Myo1p and Wsp1p, the fission yeast WASp-like protein, share functions and cooperate in controlling actin assembly. First, Myo1p localizes to cortical patches enriched at tips of growing cells and at sites of cell division. Myo1p patches partially colocalize with actin patches and are dependent on an intact actin cytoskeleton. Second, although deletion of $myo1^{+}$ is not lethal, Δmyo1 cells have actin cytoskeletal defects, including loss of polarized cell growth, delocalized actin patches, and mating defects. Third, additional disruption of $wsp1^{+}$ is synthetically lethal, suggesting that these genes may share functions. In mapping the domains of Myo1p tail that share function with Wsp1p, we discovered that a Myo1p construct with just the head and TH1 domains is sufficient for cortical localization and to rescue all Δmyo1 defects. However, it fails to rescue the Δmyo1 Δwsp1 lethality. Additional tail domains, TH2 and TH3, are required to complement the double mutant. Fourth, we show that a recombinant Myo1p tail binds to Arp2/3 complex and activates its actin nucleation activity. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 4 |
| Volume Number | 151 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 2000-11-13 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Actins Metabolism Calmodulin-Binding Proteins Cytoskeletal Proteins Fungal Proteins Myosin Heavy Chains Schizosaccharomyces Pombe Proteins Schizosaccharomyces Physiology Actin-Related Protein 2 Actin-Related Protein 3 Amino Acid Sequence Animals Cell Division Cloning, Molecular Chemistry Genetics Genetic Complementation Test Genotype Kinetics Molecular Sequence Data Myosin Type I Recombinant Fusion Proteins Recombinant Proteins Restriction Mapping Cytology Sequence Alignment Sequence Homology, Amino Acid Research Support, U.S. Gov't, P.H.S. Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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