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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Drubin, D. G. Qualmann, B. Kessels, M. M. Engqvist-goldstein, A. E. |
| Description | Author Affiliation: Kessels MM ( Department of Neurochemistry and Molecular Biology, Leibniz Institute for Neurobiology, D-39008 Magdeburg, Germany. kessels@ifn-magdeburg.de) |
| Abstract | The actin cytoskeleton has been implicated in endocytosis, yet few molecular links to the endocytic machinery have been established. Here we show that the mammalian F-actin–binding protein Abp1 (SH3P7/HIP-55) can functionally link the actin cytoskeleton to dynamin, a GTPase that functions in endocytosis. Abp1 binds directly to dynamin in vitro through its SH3 domain. Coimmunoprecipitation and colocalization studies demonstrated the in vivo relevance of this interaction. In neurons, mammalian Abp1 and dynamin colocalized at actin-rich sites proximal to the cell body during synaptogenesis. In fibroblasts, mAbp1 appeared at dynamin-rich sites of endocytosis upon growth factor stimulation. To test whether Abp1 functions in endocytosis, we overexpressed several Abp1 constructs in Cos-7 cells and assayed receptor-mediated endocytosis. While overexpression of Abp1's actin-binding modules did not interfere with endocytosis, overexpression of the SH3 domain led to a potent block of transferrin uptake. This implicates the Abp1/dynamin interaction in endocytic function. The endocytosis block was rescued by cooverexpression of dynamin. Since the addition of the actin-binding modules of Abp1 to the SH3 domain construct also fully restored endocytosis, Abp1 may support endocytosis by combining its SH3 domain interactions with cytoskeletal functions in response to signaling cascades converging on this linker protein. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 2 |
| Volume Number | 153 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 2001-04-16 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Actins Metabolism Cytoskeleton Endocytosis Physiology GTP Phosphohydrolases Microfilament Proteins Neurons 3T3 Cells Adaptor Protein Complex Alpha Subunits Adaptor Proteins, Vesicular Transport Animals Brain Chemistry COS Cells Dynamins Drug Effects Genes, Reporter Genetics Membrane Proteins Mice Chemistry Microscopy, Fluorescence Microtubules Models, Biological Platelet-Derived Growth Factor Pharmacology Protein Binding Recombinant Fusion Proteins Signal Transduction Tetradecanoylphorbol Acetate Transferrin Src Homology Domains Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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