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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Osawa, Masaki Masuda, Michitaka Fujiwara, Keigi Kusano, Ken-ichi |
| Description | Author Affiliation: Osawa M ( Department of Structural Analysis, National Cardiovascular Center Research Institute, Osaka 565-8565, Japan.) |
| Abstract | Fluid shear stress (FSS) induces many forms of responses, including phosphorylation of extracellular signal–regulated kinase (ERK) in endothelial cells (ECs). We have earlier reported rapid tyrosine phosphorylation of platelet endothelial cell adhesion molecule-1 (PECAM-1) in ECs exposed to FSS. Osmotic changes also induced similar PECAM-1 and ERK phosphorylation with nearly identical kinetics. Because both FSS and osmotic changes should mechanically perturb the cell membrane, they might activate the same mechanosignaling cascade. When PECAM-1 is tyrosine phosphorylated by FSS or osmotic changes, SHP-2 binds to it. Here we show that ERK phosphorylation by FSS or osmotic changes depends on PECAM-1 tyrosine phosphorylation, SHP-2 binding to phospho-PECAM-1, and SHP-2 phosphatase activity. In ECs under flow, detectable amounts of SHP-2 and Gab1 translocated from the cytoplasm to the EC junction. When magnetic beads coated with antibodies against the extracellular domain of PECAM-1 were attached to ECs and tugged by magnetic force for 10 min, PECAM-1 associated with the beads was tyrosine phosphorylated. ERK was also phosphorylated in these cells. Binding of the beads by itself or pulling on the cell surface using poly-l–coated beads did not induce phosphorylation of PECAM-1 and ERK. These results suggest that PECAM-1 is a mechanotransduction molecule. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 4 |
| Volume Number | 158 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 2002-08-19 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Antigens, CD31 Physiology Endothelium, Vascular Signal Transduction Stress, Mechanical Animals Cell Adhesion Cytology Enzyme Activation Intercellular Junctions Metabolism Intracellular Signaling Peptides And Proteins Mitogen-Activated Protein Kinases Osmolar Concentration Phosphoproteins Protein Transport Protein Tyrosine Phosphatase, Non-Receptor Type 11 Protein Tyrosine Phosphatases Research Support, Non-U.S. Gov't Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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