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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Randazzo, Paul A. Gao, Minggeng Premont, Richard T. Bourgoin, Sylvain Lee, Stella Y. Hsu, Victor W. Yang, Jia-shu |
| Description | Author Affiliation: Yang JS ( Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, and Department of Medicine, Harvard Medical School, Boston, MA 02115, USA.) |
| Abstract | The role of GTPase-activating protein (GAP) that deactivates ADP-ribosylation factor 1 (ARF1) during the formation of coat protein I (COPI) vesicles has been unclear. GAP is originally thought to antagonize vesicle formation by triggering uncoating, but later studies suggest that GAP promotes cargo sorting, a process that occurs during vesicle formation. Recent models have attempted to reconcile these seemingly contradictory roles by suggesting that cargo proteins suppress GAP activity during vesicle formation, but whether GAP truly antagonizes coat recruitment in this process has not been assessed directly. We have reconstituted the formation of COPI vesicles by incubating Golgi membrane with purified soluble components, and find that ARFGAP1 in the presence of GTP promotes vesicle formation and cargo sorting. Moreover, the presence of GTPγS not only blocks vesicle uncoating but also vesicle formation by preventing the proper recruitment of GAP to nascent vesicles. Elucidating how GAP functions in vesicle formation, we find that the level of GAP on the reconstituted vesicles is at least as abundant as COPI and that GAP binds directly to the dilysine motif of cargo proteins. Collectively, these findings suggest that ARFGAP1 promotes vesicle formation by functioning as a component of the COPI coat. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 1 |
| Volume Number | 159 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 2002-10-14 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | ADP-Ribosylation Factors Metabolism COP-Coated Vesicles Cell Membrane GTPase-Activating Proteins Genetics Animals CHO Cells Ultrastructure Chemistry Cricetinae Genes, Reporter Golgi Apparatus Guanosine Triphosphate Analogs & Derivatives Palmitoyl Coenzyme A Phospholipids Receptors, Peptide Recombinant Fusion Proteins Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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