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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Cremer, Marion Tashiro, Satoshi Cremer, Thomas Schermelleh, Lothar Walter, Joachim |
| Description | Author Affiliation: Walter J ( Dept. Biologie II, Lehrstuhl für Anthropologie und Humangenetik, LMU, Richard-Wagner-Strasse 10/I, 80333 München, Germany.) |
| Abstract | Whether chromosomes maintain their nuclear positions during interphase and from one cell cycle to the next has been controversially discussed. To address this question, we performed long-term live-cell studies using a HeLa cell line with GFP-tagged chromatin. Positional changes of the intensity gravity centers of fluorescently labeled chromosome territories (CTs) on the order of several μm were observed in early G1, suggesting a role of CT mobility in establishing interphase nuclear architecture. Thereafter, the positions were highly constrained within a range of ∼1 μm until the end of G2. To analyze possible changes of chromosome arrangements from one cell cycle to the next, nuclei were photobleached in G2 maintaining a contiguous zone of unbleached chromatin at one nuclear pole. This zone was stably preserved until the onset of prophase, whereas the contiguity of unbleached chromosome segments was lost to a variable extent, when the metaphase plate was formed. Accordingly, chromatin patterns observed in daughter nuclei differed significantly from the mother cell nucleus. We conclude that CT arrangements were stably maintained from mid G1 to late G2/early prophase, whereas major changes of CT neighborhoods occurred from one cell cycle to the next. The variability of CT neighborhoods during clonal growth was further confirmed by chromosome painting experiments. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 5 |
| Volume Number | 160 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 2003-03-03 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Cell Nucleus Metabolism Chromosome Segregation Genetics Chromosomes Eukaryotic Cells G1 Phase Interphase Mitosis Cell Movement Ultrastructure Clone Cells Cytology Green Fluorescent Proteins HeLa Cells Histones Luminescent Proteins Microscopy, Confocal Research Support, Non-U.S. Gov't Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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