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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Erpel, Susanne Mironov, Alexander Prusiner, Stanley B. Peters, Peter J. Peretz, David Burton, Dennis R. Dearmond, Stephen J. Van Donselaar, Elly Williamson, R. Anthony Leclerc, Estelle Vey, Martin |
| Description | Author Affiliation: Peters PJ ( Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, Plesmanlaan 121-H4, 1066 CX Amsterdam, The Netherlands. p.peters@nki.nl) |
| Abstract | To understand the posttranslational conversion of the cellular prion protein $(PrP^{C})$ to its pathologic conformation, it is important to define the intracellular trafficking pathway of $PrP^{C}$ within the endomembrane system. We studied the localization and internalization of $PrP^{C}$ in CHO cells using cryoimmunogold electron microscopy. At steady state, $PrP^{C}$ was enriched in caveolae both at the TGN and plasma membrane and in interconnecting chains of endocytic caveolae. Protein A–gold particles bound specifically to $PrP^{C}$ on live cells. These complexes were delivered via caveolae to the pericentriolar region and via nonclassical, caveolae-containing early endocytic structures to late endosomes/lysosomes, thereby bypassing the internalization pathway mediated by clathrin-coated vesicles. Endocytosed $PrP^{C}-containing$ caveolae were not directed to the ER and Golgi complex. Uptake of caveolae and degradation of $PrP^{C}$ was slow and sensitive to filipin. This caveolae-dependent endocytic pathway was not observed for several other glycosylphosphatidyl inositol (GPI)-anchored proteins. We propose that this nonclassical endocytic pathway is likely to determine the subcellular location of $PrP^{C}$ conversion. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 4 |
| Volume Number | 162 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 2003-08-18 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Caveolae Metabolism Prions Animals Bacterial Proteins CHO Cells Ultrastructure Caveolin 1 Caveolins Biosynthesis Genetics Cricetinae Endosomes Gold Colloid Microscopy, Electron Protein Transport Physiology Receptors, Transferrin Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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