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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Dragestein, Katharina A. Van Haren, Jeffrey Knoch, Tobias A. Grosveld, Frank Galjart, Niels Akhmanova, Anna Van Cappellen, Wiggert A. Tsibidis, George D. |
| Description | Author Affiliation: Dragestein KA ( Department of Cell Biology and Genetics, Erasmus Medical Center, 3000 DR Rotterdam, Netherlands.) |
| Abstract | Microtubule (MT) plus end–tracking proteins (+TIPs) specifically recognize the ends of growing MTs. +TIPs are involved in diverse cellular processes such as cell division, cell migration, and cell polarity. Although +TIP tracking is important for these processes, the mechanisms underlying plus end specificity of mammalian +TIPs are not completely understood. Cytoplasmic linker protein 170 (CLIP-170), the prototype +TIP, was proposed to bind to MT ends with high affinity, possibly by copolymerization with tubulin, and to dissociate seconds later. However, using fluorescence-based approaches, we show that two +TIPs, CLIP-170 and end-binding protein 3 (EB3), turn over rapidly on MT ends. Diffusion of CLIP-170 and EB3 appears to be rate limiting for their binding to MT plus ends. We also report that the ends of growing MTs contain a surplus of sites to which CLIP-170 binds with relatively low affinity. We propose that the observed loss of fluorescent +TIPs at plus ends does not reflect the behavior of single molecules but is a result of overall structural changes of the MT end. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 4 |
| Volume Number | 180 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 2008-02-25 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Microtubule-Associated Proteins Metabolism Microtubules Neoplasm Proteins Amino Acid Motifs Animals Binding Sites Physiology COS Cells Cercopithecus Aethiops Green Fluorescent Proteins Genetics HeLa Cells Mice Mice, Transgenic Ultrastructure Protein Binding Protein Transport Recombinant Fusion Proteins Time Factors Research Support, Non-U.S. Gov't Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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