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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Reim, Kerstin Bost, Anneka Moghadam, Paanteha K. Dutta, Soumyajit Rettig, Jens Yarzagaray, Antonio Schwarz, Yvonne Dhara, Madhurima Bruns, Dieter Mohrmann, Ralf Brose, Nils Schirra, Claudia Grabner, Chad |
| Description | Author Affiliation: Dhara M ( Institute for Physiology, University of Saarland, 66424 Homburg/Saar, Germany.) |
| Abstract | ComplexinII (CpxII) and SynaptotagminI (SytI) have been implicated in regulating the function of SNARE proteins in exocytosis, but their precise mode of action and potential interplay have remained unknown. In this paper, we show that CpxII increases $Ca^{2+}-triggered$ vesicle exocytosis and accelerates its secretory rates, providing two independent, but synergistic, functions to enhance synchronous secretion. Specifically, we demonstrate that the C-terminal domain of CpxII increases the pool of primed vesicles by hindering premature exocytosis at submicromolar $Ca^{2+}$ concentrations, whereas the N-terminal domain shortens the secretory delay and accelerates the kinetics of $Ca^{2+}-triggered$ exocytosis by increasing the $Ca^{2+}$ affinity of synchronous secretion. With its C terminus, CpxII attenuates fluctuations of the early fusion pore and slows its expansion but is functionally antagonized by SytI, enabling rapid transmitter discharge from single vesicles. Thus, our results illustrate how key features of CpxII, SytI, and their interplay transform the constitutively active SNARE-mediated fusion mechanism into a highly synchronized, $Ca^{2+}-triggered$ release apparatus. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 7 |
| Volume Number | 204 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 2014-03-31 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Adaptor Proteins, Vesicular Transport Physiology Exocytosis Nerve Tissue Proteins Animals Calcium Signaling Cells, Cultured Chromaffin Cells Secretion Chromaffin Granules Kinetics Membrane Fusion Membrane Proteins Metabolism Mice Mice, Knockout SNARE Proteins Secretory Vesicles Synaptotagmins Research Support, Non-U.S. Gov't Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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