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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bates, Mark Zhuang, Xiaowei Huang, Bo Wang, Wenqin |
| Description | Author Affiliation: Huang B ( Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.) |
| Abstract | Recent advances in far-field fluorescence microscopy have led to substantial improvements in image resolution, achieving a near-molecular resolution of 20 to 30 nanometers in the two lateral dimensions. Three-dimensional (3D) nanoscale-resolution imaging, however, remains a challenge. We demonstrated 3D stochastic optical reconstruction microscopy (STORM) by using optical astigmatism to determine both axial and lateral positions of individual fluorophores with nanometer accuracy. Iterative, stochastic activation of photoswitchable probes enables high-precision 3D localization of each probe, and thus the construction of a 3D image, without scanning the sample. Using this approach, we achieved an image resolution of 20 to 30 nanometers in the lateral dimensions and 50 to 60 nanometers in the axial dimension. This development allowed us to resolve the 3D morphology of nanoscopic cellular structures. |
| ISSN | 00368075 |
| e-ISSN | 10959203 |
| Journal | Science |
| Issue Number | 5864 |
| Volume Number | 319 |
| Language | English |
| Publisher | American Association for the Advancement of Science (United States) |
| Publisher Date | 2008-02-08 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Coated Pits, Cell-Membrane Ultrastructure Imaging, Three-Dimensional Microscopy, Fluorescence Microtubules Nanotechnology Animals Carbocyanines Cell Line Cercopithecus Aethiops Clathrin Cyclic AMP Analogs & Derivatives Fluorescent Antibody Technique Fluorescent Dyes Image Processing, Computer-Assisted Instrumentation Quantum Dots Stochastic Processes Streptavidin Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | History and Philosophy of Science |
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