NDLI: Generation of mouse induced pluripotent stem cells without viral vectors.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hyenjong, Hong Yamanaka, Shinya Nakagawa, Masato Okita, Keisuke Ichisaka, Tomoko
Description	Author Affiliation: Okita K ( Center for iPS Cell Research and Application (CiRA), Institute for Integrated Cell-Material Sciences, Kyoto University, Kyoto 606-8507, Japan.)
Abstract	Induced pluripotent stem (iPS) cells have been generated from mouse and human somatic cells by introducing Oct3/4 and Sox2 with either Klf4 and c-Myc or Nanog and Lin28 using retroviruses or lentiviruses. Patient-specific iPS cells could be useful in drug discovery and regenerative medicine. However, viral integration into the host genome increases the risk of tumorigenicity. Here, we report the generation of mouse iPS cells without viral vectors. Repeated transfection of two expression plasmids, one containing the complementary DNAs (cDNAs) of Oct3/4, Sox2, and Klf4 and the other containing the c-Myc cDNA, into mouse embryonic fibroblasts resulted in iPS cells without evidence of plasmid integration, which produced teratomas when transplanted into mice and contributed to adult chimeras. The production of virus-free iPS cells, albeit from embryonic fibroblasts, addresses a critical safety concern for potential use of iPS cells in regenerative medicine.
ISSN	00368075
Issue Number	5903
Volume Number	322
e-ISSN	10959203
Journal	Science
Language	English
Publisher	American Association for the Advancement of Science (United States)
Publisher Date	2008-11-07
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cellular Reprogramming Fibroblasts Cytology Plasmids Pluripotent Stem Cells Transfection Animals Chimera DNA, Complementary Embryo, Mammalian Embryonic Stem Cells Metabolism Genes, Myc Genetic Vectors Kruppel-Like Transcription Factors Genetics Mice Octamer Transcription Factor-3 Transplantation Retroviridae SOXB1 Transcription Factors Teratoma Etiology Journal Article Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	History and Philosophy of Science

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