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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Lin, Michael Z. Zhou, Xin X. Lam, Amy J. Chung, Hokyung K. |
| Description | Author Affiliation: Zhou XX ( Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.) |
| Abstract | Fluorescent proteins (FPs) are widely used as optical sensors, whereas other light-absorbing domains have been used for optical control of protein localization or activity. Here, we describe light-dependent dissociation and association in a mutant of the photochromic FP Dronpa, and we used it to control protein activities with light. We created a fluorescent light-inducible protein design in which Dronpa domains are fused to both termini of an enzyme domain. In the dark, the Dronpa domains associate and cage the protein, but light induces Dronpa dissociation and activates the protein. This method enabled optical control over guanine nucleotide exchange factor and protease domains without extensive screening. Our findings extend the applications of FPs from exclusively sensing functions to also encompass optogenetic control. |
| ISSN | 00368075 |
| e-ISSN | 10959203 |
| Journal | Science |
| Issue Number | 6108 |
| Volume Number | 338 |
| Language | English |
| Publisher | American Association for the Advancement of Science (United States) |
| Publisher Date | 2012-11-09 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Luminescent Proteins Chemistry Protein Structure, Tertiary Recombinant Fusion Proteins Adaptor Proteins, Vesicular Transport Genetics Metabolism Animals Cell Membrane Fluorescence HeLa Cells Mice Models, Molecular NIH 3T3 Cells Native Polyacrylamide Gel Electrophoresis Optogenetics Protein Conformation Protein Engineering Protein Multimerization Pseudopodia Ultrastructure Serine Endopeptidases Viral Nonstructural Proteins Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | History and Philosophy of Science |
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