NDLI: A sentinel goblet cell guards the colonic crypt by triggering Nlrp6-dependent Muc2 secretion.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Johansson, Malin E. V. Nyström, Elisabeth E. L. Birchenough, George M. H. Hansson, Gunnar C.
Description	Author Affiliation: Birchenough GM ( Department of Medical Biochemistry, University of Gothenburg, 40530 Gothenburg, Sweden.); Nyström EE ( Department of Medical Biochemistry, University of Gothenburg, 40530 Gothenburg, Sweden.); Johansson ME ( Department of Medical Biochemistry, University of Gothenburg, 40530 Gothenburg, Sweden.); Hansson GC ( Department of Medical Biochemistry, University of Gothenburg, 40530 Gothenburg, Sweden. gunnar.hansson@medkem.gu.se.)
Abstract	Innate immune signaling pathways contribute to the protection of host tissue when bacterially challenged. Colonic goblet cells are responsible for generating the two mucus layers that physically separate the luminal microbiota from the host epithelium. Analysis of colonic tissues from multiple mouse strains allowed us to identify a 'sentinel' goblet cell (senGC) localized to the colonic crypt entrance. This cell nonspecifically endocytoses and reacts to the TLR2/1, TLR4, and TLR5 ligands by activating the Nlrp6 inflammasome downstream of TLR- and MyD88-dependent Nox/Duox reactive oxygen species synthesis. This triggers calcium ion-dependent compound exocytosis of Muc2 mucin from the senGC and generates an intercellular gap junction signal; in turn, this signal induces Muc2 secretion from adjacent goblet cells in the upper crypt, which expels bacteria. Thus, senGCs guard and protect the colonic crypt from bacterial intruders that have penetrated the inner mucus layer.
ISSN	00368075
Issue Number	6293
Volume Number	352
e-ISSN	10959203
Journal	Science
Language	English
Publisher	American Association for the Advancement of Science (United States)
Publisher Date	2016-06-24
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Colon Immunology Microbiology Gastrointestinal Microbiome Goblet Cells Mucin-2 Metabolism Receptors, Cell Surface Animals Bacteria Calcium Endocytosis Immunity, Innate Inflammasomes Intestinal Mucosa Ligands Mice Mice, Inbred C57BL Myeloid Differentiation Factor 88 Reactive Oxygen Species Genetics Signal Transduction Toll-Like Receptor 2 Toll-Like Receptor 4 Toll-Like Receptor 5 Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	History and Philosophy of Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in