| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Racek, Tomas Somech, Raz Lev, Atar Babushkin, Tatiana Carrington, Blake Soudack, Michalle Barshack, Iris Toren, Amos Avivi, Camila Klein, Christoph Bishop, Kevin Mullikin, James Vilboux, Thierry Huizing, Marjan Van Der Werff Ten Bosch, Jutte Schiby, Ginette Malicdan, May Christine V. Järvinen, Päivi Atawneh, Osama M. Wolach, Baruch Anikster, Yair Cullinane, Andrew Gahl, William A. Amariglio, Ninette Puchalka, Jacek Garty, Ben Zion Gavrieli, Ronit Polak-charcon, Sylvie Sood, Raman Simon, Amos J. Eyal, Eran Rechavi, Gideon |
| Description |
Author Affiliation: Vilboux T ( Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.) |
| Abstract |
BACKGROUND: Neutrophils are the predominant phagocytes that provide protection against bacterial and fungal infections. Genetically determined neutrophil disorders confer a predisposition to severe infections and reveal novel mechanisms that control vesicular trafficking, hematopoiesis, and innate immunity. METHODS: We clinically evaluated seven children from five families who had neutropenia, neutrophil dysfunction, bone marrow fibrosis, and nephromegaly. To identify the causative gene, we performed homozygosity mapping using single-nucleotide polymorphism arrays, whole-exome sequencing, immunoblotting, immunofluorescence, electron microscopy, a real-time quantitative polymerase-chain-reaction assay, immunohistochemistry, flow cytometry, fibroblast motility assays, measurements of apoptosis, and zebrafish models. Correction experiments were performed by transfecting mutant fibroblasts with the nonmutated gene. RESULTS: All seven affected children had homozygous mutations (Thr224Asn or Glu238Lys, depending on the child's ethnic origin) in VPS45, which encodes a protein that regulates membrane trafficking through the endosomal system. The level of VPS45 protein was reduced, as were the VPS45 binding partners rabenosyn-5 and syntaxin-16. The level of ß1 integrin was reduced on the surface of VPS45-deficient neutrophils and fibroblasts. VPS45-deficient fibroblasts were characterized by impaired motility and increased apoptosis. A zebrafish model of vps45 deficiency showed a marked paucity of myeloperoxidase-positive cells (i.e., neutrophils). Transfection of patient cells with nonmutated VPS45 corrected the migration defect and decreased apoptosis. CONCLUSIONS: Defective endosomal intracellular protein trafficking due to biallelic mutations in VPS45 underlies a new immunodeficiency syndrome involving impaired neutrophil function. (Funded by the National Human Genome Research Institute and others.). |
| ISSN |
00284793 |
| e-ISSN |
15334406 |
| Journal |
New England Journal of Medicine |
| Issue Number |
1 |
| Volume Number |
369 |
| Language |
English |
| Publisher |
Massachusetts Medical Society (United States) |
| Publisher Date |
2013-07-04 |
| Publisher Place |
United States |
| Access Restriction |
Open |
| Subject Keyword |
Immunologic Deficiency Syndromes Genetics Neutropenia Congenital Vesicular Transport Proteins Animals Endosomes Metabolism Homozygote Immunology Mutation Neutrophils Physiology Phenotype Protein Transport Zebrafish Research Support, N.I.H., Intramural Research Support, Non-U.S. Gov't Medicine |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Medicine |
