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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Christendat, Dinesh Zarembinski, Thomas Singh, Sasha Collart, Frank Joachimiak, Andrzej Korolev, Sergey Koroleva, Olga |
| Description | Author Affiliation: Singh S ( Department of Botany, University of Toronto, Toronto, Ontario M5S 3B2, Canada.) |
| Abstract | To date two classes of shikimate dehydrogenases have been identified and characterized, YdiB and AroE. YdiB is a bifunctional enzyme that catalyzes the reversible reductions of dehydroquinate to quinate and dehydroshikimate to shikimate in the presence of either NADH or NADPH. In contrast, AroE catalyzes the reversible reduction of dehydroshikimate to shikimate in the presence of NADPH. Here we report the crystal structure and biochemical characterization of HI0607, a novel class of shikimate dehydrogenase annotated as shikimate dehydrogenase-like. The kinetic properties of HI0607 are remarkably different from those of AroE and YdiB. In comparison with YdiB, HI0607 catalyzes the oxidation of shikimate but not quinate. The turnover rate for the oxidation of shikimate is approximately 1000-fold lower compared with that of AroE. Phylogenetic analysis reveals three independent clusters representing three classes of shikimate dehydrogenases, namely AroE, YdiB, and this newly characterized shikimate dehydrogenase-like protein. In addition, mutagenesis studies of two invariant residues, Asp-103 and Lys-67, indicate that they are important catalytic groups that may function as a catalytic pair in the shikimate dehydrogenase reaction. This is the first study that describes the crystal structure as well as mutagenesis and mechanistic analysis of this new class of shikimate dehydrogenase. |
| ISSN | 00219258 |
| e-ISSN | 1083351X |
| Journal | Journal of Biological Chemistry |
| Issue Number | 17 |
| Volume Number | 280 |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology (United States) |
| Publisher Date | 2005-04-29 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Alcohol Oxidoreductases Chemistry Genetics Haemophilus Influenzae Enzymology Asparagine Bayes Theorem Catalysis Circular Dichroism Cloning, Molecular Crystallography, X-Ray Herbicides Hydrogen-Ion Concentration Ions Kinetics Lysine Models, Molecular Mutagenesis Mutagenesis, Site-Directed NAD NADP Oxygen Phylogeny Protein Conformation Protein Structure, Secondary Protein Structure, Tertiary Substrate Specificity Temperature Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Biochemistry Molecular Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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