NDLI: Control of cellular physiology by TM9 proteins in yeast and Dictyostelium.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Mösch, Hans-ulrich Cosson, Pierre Letourneur, François Froquet, Romain De Virgilio, Claudio Birke, Raphael Cherix, Nathalie Cameroni, Elisabetta Benghezal, Mohammed
Description	Author Affiliation: Froquet R ( Département de Physiologie et Métabolisme Cellulaire, Centre Médical Universitaire, Université de Genève, rue Michel Servet 1, CH-1211 Genève 4, Switzerland.)
Abstract	TM9 proteins constitute a well defined family, characterized by the presence of a large variable extracellular domain and nine putative transmembrane domains. This family is highly conserved throughout evolution and comprises three members in Dictyostelium discoideum and Saccharomyces cerevisiae and four in humans and mice. In Dictyostelium, previous analysis demonstrated that TM9 proteins are implicated in cellular adhesion. In this study, we generated TM9 mutants in S. cerevisiae and analyzed their phenotype with particular attention to cellular adhesion. S. cerevisiae strains lacking any one of the three TM9 proteins were severely suppressed for adhesive growth and filamentous growth under conditions of nitrogen starvation. In these mutants, expression of the FLO11-lacZ reporter gene was strongly reduced, whereas expression of FRE(Ty1)-lacZ was not, suggesting that TM9 proteins are implicated at a late stage of nutrient-controlled signaling pathways. We also reexamined the phenotype of Dictyostelium TM9 mutant cells, focusing on nutrient-controlled cellular functions. Although the initiation of multicellular development and autophagy was unaltered in Dictyostelium TM9 mutants, nutrient-controlled secretion of lysosomal enzymes was dysregulated in these cells. These results suggest that in both yeast and amoebae, TM9 proteins participate in the control of specific cellular functions in response to changing nutrient conditions.
ISSN	00219258
e-ISSN	1083351X
Journal	Journal of Biological Chemistry
Issue Number	11
Volume Number	283
Language	English
Publisher	American Society for Biochemistry and Molecular Biology (United States)
Publisher Date	2008-03-14
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Dictyostelium Metabolism Fungal Proteins Physiology Gene Expression Regulation, Fungal Gene Expression Regulation Membrane Proteins Animals Autophagy Cell Adhesion Genetics Lysosomes Models, Biological Mutation Plasmids Saccharomyces Cerevisiae Signal Transduction Species Specificity Research Support, Non-U.S. Gov't Biochemistry Molecular Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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