NDLI: Heme Regulatory Motifs in Heme Oxygenase-2 Form a Thiol/Disulfide Redox Switch That Responds to the Cellular Redox $State^{*}$

Please wait, while we are loading the content...

Year: 2017 Volume: 292

Year: 2016 Volume: 291

Year: 2015 Volume: 290

Year: 2014 Volume: 289

Year: 2013 Volume: 288

Year: 2012 Volume: 287

Year: 2011 Volume: 286

Year: 2010 Volume: 285

Year: 2009 Volume: 284

Year: 2009 Volume: 284 Issue: 52

Year: 2009 Volume: 284 Issue: 51

Year: 2009 Volume: 284 Issue: 50

Year: 2009 Volume: 284 Issue: 49

Year: 2009 Volume: 284 Issue: 48

Year: 2009 Volume: 284 Issue: 47

Year: 2009 Volume: 284 Issue: 46

Year: 2009 Volume: 284 Issue: 45

Year: 2009 Volume: 284 Issue: 44

Year: 2009 Volume: 284 Issue: 43

Year: 2009 Volume: 284 Issue: 42

Year: 2009 Volume: 284 Issue: 41

Year: 2009 Volume: 284 Issue: 40

Year: 2009 Volume: 284 Issue: 39

Year: 2009 Volume: 284 Issue: 38

Year: 2009 Volume: 284 Issue: 37

Year: 2009 Volume: 284 Issue: 36

Year: 2009 Volume: 284 Issue: 35

Year: 2009 Volume: 284 Issue: 34

Year: 2009 Volume: 284 Issue: 33

Year: 2009 Volume: 284 Issue: 32

Year: 2009 Volume: 284 Issue: 31

Converse conformational control of smoothened activity by structurally related small molecules.

ENaC at the Cutting Edge: Regulation of Epithelial Sodium Channels by Proteases

Exosite determinants of serpin specificity.

Roles of human AND-1 in chromosome transactions in S phase.

Thrombin Promotes Release of ATP from Lung Epithelial Cells through Coordinated Activation of Rho- and $Ca^{2+}-dependent$ Signaling Pathways

NMR conformational and dynamic consequences of a gly to ser substitution in an osteogenesis imperfecta collagen model peptide.

Farnesylation of pex19p is required for its structural integrity and function in peroxisome biogenesis.

EKLF/KLF1 controls cell cycle entry via direct regulation of E2f2.

Interferon-lambda is functionally an interferon but structurally related to the interleukin-10 family.

Determination of key residues for catalysis and RNA cleavage specificity: one mutation turns RNase II into a 'SUPER-ENZYME'.

Stress-dependent Daxx-CHIP interaction suppresses the p53 apoptotic program.

Regulatory subunit I-controlled protein kinase A activity is required for apical bile canalicular lumen development in hepatocytes.

Heme Regulatory Motifs in Heme Oxygenase-2 Form a Thiol/Disulfide Redox Switch That Responds to the Cellular Redox $State^{*}$

Direct selection of monoclonal phosphospecific antibodies without prior phosphoamino acid mapping.

X-linked inhibitor of apoptosis protein (XIAP) regulates PTEN ubiquitination, content, and compartmentalization.

Is the redox state of the ci heme of the cytochrome b6f complex dependent on the occupation and structure of the Qi site and vice versa?

Inhibition of tau polymerization with a cyanine dye in two distinct model systems.

Improved virus neutralization by plant-produced anti-HIV antibodies with a homogeneous beta1,4-galactosylated N-glycan profile.

Occludin phosphorylation and ubiquitination regulate tight junction trafficking and vascular endothelial growth factor-induced permeability.

Dependence of STIM1/Orai1-mediated Calcium Entry on Plasma Membrane Phosphoinositides

Essential role of cofilin-1 in regulating thrombin-induced RelA/p65 nuclear translocation and intercellular adhesion molecule 1 (ICAM-1) expression in endothelial cells.

LFA-1 regulates CD8+ T cell activation via T cell receptor-mediated and LFA-1-mediated Erk1/2 signal pathways.

Structural and Functional Analysis of Campylobacter jejuni PseG: A UDP-SUGAR HYDROLASE FROM THE PSEUDAMINIC ACID BIOSYNTHETIC PATHWAY

Plasminogen activator inhibitor-1 regulates integrin alphavbeta3 expression and autocrine transforming growth factor beta signaling.

Cab45b, a Munc18b-interacting partner, regulates exocytosis in pancreatic beta-cells.

Transcriptional regulation of the novel Toll-like receptor Tlr13.

Asymmetric amino acid activation by class II histidyl-tRNA synthetase from Escherichia coli.

Polycystin-1 C-terminal Cleavage Is Modulated by Polycystin-2 Expression

Mycobacterium marinum lipooligosaccharides are unique caryophyllose-containing cell wall glycolipids that inhibit tumor necrosis factor-alpha secretion in macrophages.

An Essential Role for the Plasmodium Nek-2 Nima-related Protein Kinase in the Sexual Development of Malaria $Parasites^{*}$

Aquifex aeolicus tRNA (N2,N2-guanine)-dimethyltransferase (Trm1) catalyzes transfer of methyl groups not only to guanine 26 but also to guanine 27 in tRNA.

Sirtuin 1 Functionally and Physically Interacts with Disruptor of Telomeric Silencing-1 to Regulate α-ENaC Transcription in Collecting Duct

Phosphorylated Tau interacts with c-Jun N-terminal kinase-interacting protein 1 (JIP1) in Alzheimer disease.

Specific interaction between tomato HsfA1 and HsfA2 creates hetero-oligomeric superactivator complexes for synergistic activation of heat stress gene expression.

The Biological Activity of the Prototypic Cyclotide Kalata B1 Is Modulated by the Formation of Multimeric Pores

Kruppel-like factor 2 inhibits hypoxia-inducible factor 1alpha expression and function in the endothelium.

Combinatorial Transcription Factor Regulation of the Cyclic AMP-response Element on the Pgc-1α Promoter in White 3T3-L1 and Brown HIB-1B Preadipocytes

Calmodulin Activation Limits the Rate of KCNQ2 $K^{+}$ Channel Exit from the Endoplasmic Reticulum

Red Fluorescent Protein pH Biosensor to Detect Concentrative Nucleoside Transport

Prostate-specific Klf6 inactivation impairs anterior prostate branching morphogenesis through increased activation of the Shh pathway.

Keratinocyte-derived chemokine in obesity: expression, regulation, and role in adipose macrophage infiltration and glucose homeostasis.

Protein-tyrosine Phosphatase-α and Src Functionally Link Focal Adhesions to the Endoplasmic Reticulum to Mediate Interleukin-1-induced $Ca^{2+}$ Signaling

The magnitude of the light-induced conformational change in different rhodopsins correlates with their ability to activate G proteins.

DLX5 (distal-less homeobox 5) promotes tumor cell proliferation by transcriptionally regulating MYC.

Interaction of antithrombin with sulfated, low molecular weight lignins: opportunities for potent, selective modulation of antithrombin function.

Intramolecular isopeptide bonds give thermodynamic and proteolytic stability to the major pilin protein of Streptococcus pyogenes.

Biochemical and functional characterization of six SIX1 Branchio-oto-renal syndrome mutations.

Molecular Mechanism Underlying RAG1/RAG2 Synaptic Complex Formation

NaKtide, a Na/K-ATPase-derived peptide Src inhibitor, antagonizes ouabain-activated signal transduction in cultured cells.

Arachidonic Acid Stimulates Cell Adhesion through a Novel p38 MAPK-RhoA Signaling Pathway That Involves Heat Shock Protein 27

Enhanced cytoprotective effects of the inhibitor of apoptosis protein cellular IAP1 through stabilization with TRAF2.

Differential Phosphorylation of Plant Translation Initiation Factors by Arabidopsis thaliana CK2 Holoenzymes

Fungal zymosan and mannan activate the cryopyrin inflammasome.

Human mitochondrial SUV3 and polynucleotide phosphorylase form a 330-kDa heteropentamer to cooperatively degrade double-stranded RNA with a 3'-to-5' directionality.

Molecular requirements for recognition of brain voltage-gated sodium channels by scorpion alpha-toxins.

Dual 12/15- and 5-lipoxygenase deficiency in macrophages alters arachidonic acid metabolism and attenuates peritonitis and atherosclerosis in ApoE knock-out mice.

Phosphorylation of Plant Translation Initiation Factors by CK2 Enhances the in Vitro Interaction of Multifactor Complex Components

Calcium Elevation in Mitochondria Is the Main $Ca^{2+}$ Requirement for Mitochondrial Permeability Transition Pore (mPTP) $Opening^{*}$

Conformational changes during nucleotide selection by Sulfolobus solfataricus DNA polymerase Dpo4.

Requirement of cell cycle and apoptosis regulator 1 for target gene activation by Wnt and beta-catenin and for anchorage-independent growth of human colon carcinoma cells.

B cell lymphoma (Bcl)-2 protein is the major determinant in bcl-2 adenine-uridine-rich element turnover overcoming HuR activity.

Degradation of sterol regulatory element-binding protein precursor requires the endoplasmic reticulum-associated degradation components Ubc7 and Hrd1 in fission yeast.

Scavenger chemokine (CXC motif) receptor 7 (CXCR7) is a direct target gene of HIC1 (hypermethylated in cancer 1).

Chaperone-mediated $Cu^{+}$ Delivery to $Cu^{+}$ Transport ATPases: REQUIREMENT OF NUCLEOTIDE BINDING

Nuclear export of the yeast hexokinase 2 protein requires the Xpo1 (Crm1)-dependent pathway.

Mismatch recognition protein MutSbeta does not hijack (CAG)n hairpin repair in vitro.

Peptidoglycan induces cyclooxygenase-2 expression in macrophages by activating the neutral sphingomyelinase-ceramide pathway.

Direct Bre1-Paf1 Complex Interactions and RING Finger-independent Bre1-Rad6 Interactions Mediate Histone H2B Ubiquitylation in Yeast

Structure and functional implications of the human rad9-hus1-rad1 cell cycle checkpoint complex.

Role of lactate in lipid metabolism, just always inhibiting lipolysis?

The ATPase activity of BfpD is greatly enhanced by zinc and allosteric interactions with other Bfp proteins.

Year: 2009 Volume: 284 Issue: 30

Year: 2009 Volume: 284 Issue: 29

Year: 2009 Volume: 284 Issue: 28

Year: 2009 Volume: 284 Issue: 27

Year: 2009 Volume: 284 Issue: 26

Year: 2009 Volume: 284 Issue: 25

Year: 2009 Volume: 284 Issue: 24

Year: 2009 Volume: 284 Issue: 23

Year: 2009 Volume: 284 Issue: 22

Year: 2009 Volume: 284 Issue: 21

Year: 2009 Volume: 284 Issue: 20

Year: 2009 Volume: 284 Issue: 19

Year: 2009 Volume: 284 Issue: 18

Year: 2009 Volume: 284 Issue: 17

Year: 2009 Volume: 284 Issue: 16

Year: 2009 Volume: 284 Issue: 15

Year: 2009 Volume: 284 Issue: 14

Year: 2009 Volume: 284 Issue: 13

Year: 2009 Volume: 284 Issue: 12

Year: 2009 Volume: 284 Issue: 11

Year: 2009 Volume: 284 Issue: 10

Year: 2009 Volume: 284 Issue: 9

Year: 2009 Volume: 284 Issue: 8

Year: 2009 Volume: 284 Issue: 7

Year: 2009 Volume: 284 Issue: 6

Year: 2009 Volume: 284 Issue: 5

Year: 2009 Volume: 284 Issue: 4

Year: 2009 Volume: 284 Issue: 3

Year: 2009 Volume: 284 Issue: 2

Year: 2009 Volume: 284 Issue: 1

Year: 2008 Volume: 283

Year: 2007 Volume: 282

Year: 2006 Volume: 281

Year: 2005 Volume: 280

Year: 2004 Volume: 279

Year: 2003 Volume: 278

Year: 2002 Volume: 277

Year: 2001 Volume: 276

Year: 2000 Volume: 275

Year: 1999 Volume: 274

Year: 1998 Volume: 273

Year: 1997 Volume: 272

Year: 1996 Volume: 271

Year: 1995 Volume: 270

Year: 1994 Volume: 269

Year: 1993 Volume: 268

Year: 1992 Volume: 267

Year: 1991 Volume: 266

Year: 1990 Volume: 265

Year: 1989 Volume: 264

Year: 1988 Volume: 263

Year: 1987 Volume: 262

Year: 1986 Volume: 261

Year: 1984 Volume: 259

Year: 1982 Volume: 257

Year: 1981 Volume: 256

Year: 1975 Volume: 250

Heme Regulatory Motifs in Heme Oxygenase-2 Form a Thiol/Disulfide Redox Switch That Responds to the Cellular Redox $State^{*}$

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Leichert, Lars I. Jenkins, Paul M. Martens, Jeffrey R. Yi, Li Jakob, Ursula Ragsdale, Stephen W.
Description	Author Affiliation: Yi L ( Departments of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA.)
Abstract	Heme oxygenase (HO) catalyzes the rate-limiting step in heme catabolism to generate CO, biliverdin, and free iron. Two isoforms of HO have been identified in mammals: inducible HO-1 and constitutively expressed HO-2. HO-1 and HO-2 share similar physical and kinetic properties but have different physiological roles and tissue distributions. Unlike HO-1, which lacks cysteine residues, HO-2 contains three Cys-Pro signatures, known as heme regulatory motifs (HRMs), which are known to control processes related to iron and oxidative metabolism in organisms from bacteria to humans. In HO-2, the C-terminal HRMs constitute a thiol/disulfide redox switch that regulates affinity of the enzyme for heme (Yi, L., and Ragsdale, S. W. (2007) J. Biol. Chem. 282, 20156–21067). Here, we demonstrate that the thiol/disulfide switch in human HO-2 is physiologically relevant. Its redox potential was measured to be −200 mV, which is near the ambient intracellular redox potential. We expressed HO-2 in bacterial and human cells and measured the redox state of the C-terminal HRMs in growing cells by thiol-trapping experiments using the isotope-coded affinity tag technique. Under normal growth conditions, the HRMs are 60–70% reduced, whereas oxidative stress conditions convert most (86–89%) of the HRMs to the disulfide state. Treatment with reductants converts the HRMs largely (81–87%) to the reduced dithiol state. Thus, the thiol/disulfide switch in HO-2 responds to cellular oxidative stress and reductive conditions, representing a paradigm for how HRMs can integrate heme homeostasis with CO signaling and redox regulation of cellular metabolism.
ISSN	00219258
e-ISSN	1083351X
Journal	Journal of Biological Chemistry
Issue Number	31
Volume Number	284
Language	English
Publisher	American Society for Biochemistry and Molecular Biology (United States)
Publisher Date	2009-07-31
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Disulfides Metabolism Heme Oxygenase (Decyclizing) Chemistry Heme Sulfhydryl Compounds Amino Acid Motifs Cell Line Escherichia Coli Isolation & Purification Mass Spectrometry Oxidation-Reduction Research Support, N.I.H., Extramural Biochemistry Molecular Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in