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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zaccardi, Margot J. Yezdimer, Eric M. Boehr, David D. |
| Description | Author Affiliation: Zaccardi MJ ( From the Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania 16802.) |
| Abstract | The tryptophan biosynthetic enzyme indole-3-glycerol phosphate synthase is a proposed target for new antimicrobials and is a favored starting framework in enzyme engineering studies. Forty years ago, Parry proposed that the enzyme mechanism proceeds through two intermediates in a series of condensation, decarboxylation, and dehydration steps. X-ray crystal structures have suggested that Lys-110 (numbering according to the Sulfolobus solfataricus enzyme) behaves as a general acid both in the condensation and dehydration steps, but did not reveal an efficient pathway for the reprotonation of this critical residue. Our mutagenesis and kinetic experiments suggest an alternative mechanism whereby Lys-110 acts as a general acid in the condensation step, but another invariant residue, Lys-53, acts as the general acid in the dehydration step. These studies also indicate that the conserved residue Glu-51 acts as the general base in the dehydration step. The revised mechanism effectively divides the active site into discrete regions where the catalytic surfaces containing Lys-110 and Lys-53/Glu-51 catalyze the ring closure (i.e. condensation and decarboxylation) and dehydration steps, respectively. These results can be leveraged toward the development of novel inhibitors against this validated antimicrobial target and toward the rational engineering of the enzyme to produce indole derivatives that are highly prized by the pharmaceutical and agricultural industries. |
| ISSN | 00219258 |
| e-ISSN | 1083351X |
| Journal | Journal of Biological Chemistry |
| Issue Number | 37 |
| Volume Number | 288 |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology (United States) |
| Publisher Date | 2013-09-13 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Catalytic Domain Indole-3-Glycerol-Phosphate Synthase Chemistry Anti-Bacterial Agents Catalysis Crystallography, X-Ray Escherichia Coli Enzymology Glutamic Acid Lysine Mutagenesis, Site-Directed Protein Engineering Solvents Tryptophan Viscosity Research Support, U.S. Gov't, Non-P.H.S. Biochemistry Molecular Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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