NDLI: Amino Acids Activate Mammalian Target of Rapamycin (mTOR) Complex 1 without Changing Rag GTPase Guanyl Nucleotide $Charging^{*}$

Please wait, while we are loading the content...

Year: 2017 Volume: 292

Year: 2016 Volume: 291

Year: 2015 Volume: 290

Year: 2014 Volume: 289

Year: 2014 Volume: 289 Issue: 52

Year: 2014 Volume: 289 Issue: 51

Year: 2014 Volume: 289 Issue: 50

Year: 2014 Volume: 289 Issue: 49

Year: 2014 Volume: 289 Issue: 48

Year: 2014 Volume: 289 Issue: 47

Year: 2014 Volume: 289 Issue: 46

Year: 2014 Volume: 289 Issue: 45

Year: 2014 Volume: 289 Issue: 44

Year: 2014 Volume: 289 Issue: 43

Year: 2014 Volume: 289 Issue: 42

Year: 2014 Volume: 289 Issue: 41

Year: 2014 Volume: 289 Issue: 40

Year: 2014 Volume: 289 Issue: 39

Year: 2014 Volume: 289 Issue: 38

Year: 2014 Volume: 289 Issue: 37

Year: 2014 Volume: 289 Issue: 36

Year: 2014 Volume: 289 Issue: 35

Year: 2014 Volume: 289 Issue: 34

Year: 2014 Volume: 289 Issue: 33

Year: 2014 Volume: 289 Issue: 32

Year: 2014 Volume: 289 Issue: 31

Year: 2014 Volume: 289 Issue: 30

Year: 2014 Volume: 289 Issue: 29

Year: 2014 Volume: 289 Issue: 28

Year: 2014 Volume: 289 Issue: 27

Year: 2014 Volume: 289 Issue: 26

Year: 2014 Volume: 289 Issue: 25

Year: 2014 Volume: 289 Issue: 24

Year: 2014 Volume: 289 Issue: 23

Year: 2014 Volume: 289 Issue: 22

Year: 2014 Volume: 289 Issue: 21

Year: 2014 Volume: 289 Issue: 20

Year: 2014 Volume: 289 Issue: 19

Year: 2014 Volume: 289 Issue: 18

Year: 2014 Volume: 289 Issue: 17

Year: 2014 Volume: 289 Issue: 16

Year: 2014 Volume: 289 Issue: 15

Year: 2014 Volume: 289 Issue: 14

Year: 2014 Volume: 289 Issue: 13

Year: 2014 Volume: 289 Issue: 12

Year: 2014 Volume: 289 Issue: 11

Year: 2014 Volume: 289 Issue: 10

Year: 2014 Volume: 289 Issue: 9

Year: 2014 Volume: 289 Issue: 8

Year: 2014 Volume: 289 Issue: 7

Year: 2014 Volume: 289 Issue: 6

Year: 2014 Volume: 289 Issue: 5

Genetic signatures of HIV-1 envelope-mediated bystander apoptosis.

A novel non-canonical forkhead-associated (FHA) domain-binding interface mediates the interaction between Rad53 and Dbf4 proteins.

Crystal Structure of the Tetrameric Fibrinogen-like Recognition Domain of Fibrinogen C Domain Containing 1 (FIBCD1) $Protein^{*}$

Phosphorylation of CEACAM1 molecule by calmodulin kinase IID in a three-dimensional model of mammary gland lumen formation.

A novel acidic matrix protein, PfN44, stabilizes magnesium calcite to inhibit the crystallization of aragonite.

Down-modulation of SEL1L, an unfolded protein response and endoplasmic reticulum-associated degradation protein, sensitizes glioma stem cells to the cytotoxic effect of valproic acid.

The multidrug resistance IncA/C transferable plasmid encodes a novel domain-swapped dimeric protein-disulfide isomerase.

Hepatic Apolipoprotein M (ApoM) Overexpression Stimulates Formation of Larger ApoM/Sphingosine 1-Phosphate-enriched Plasma High Density Lipoprotein

Engineering Hydrogen Gas Production from Formate in a Hyperthermophile by Heterologous Production of an 18-Subunit Membrane-bound Complex

Binding of HIV-1 Vpr protein to the human homolog of the yeast DNA repair protein RAD23 (hHR23A) requires its xeroderma pigmentosum complementation group C binding (XPCB) domain as well as the ubiquitin-associated 2 (UBA2) domain.

Constitutive and nitrogen catabolite repression-sensitive production of Gat1 isoforms.

Role of the LEXE Motif of Protein-primed DNA Polymerases in the Interaction with the Incoming Nucleotide

Cognitive deficits and disruption of neurogenesis in a mouse model of apolipoprotein E4 domain interaction.

A C4-oxidizing lytic polysaccharide monooxygenase cleaving both cellulose and cello-oligosaccharides.

First evidence for a covalent linkage between enterobacterial common antigen and lipopolysaccharide in Shigella sonnei phase II ECALPS.

The Molecular Chaperone Hsp70 Activates Protein Phosphatase 5 (PP5) by Binding the Tetratricopeptide Repeat (TPR) Domain

The D-ring, not the A-ring, rotates in Synechococcus OS-B' phytochrome.

Casein kinase 1 regulates sterol regulatory element-binding protein (SREBP) to control sterol homeostasis.

The large Hsp70 Grp170 binds to unfolded protein substrates in vivo with a regulation distinct from conventional Hsp70s.

Organization of the mitochondrial apoptotic BAK pore: oligomerization of the BAK homodimers.

Dynamic structural changes underpin photoconversion of a blue/green cyanobacteriochrome between its dark and photoactivated states.

Chondroitin sulfate proteoglycan tenascin-R regulates glutamate uptake by adult brain astrocytes.

Tumor necrosis factor and transforming growth factor ß regulate clock genes by controlling the expression of the cold inducible RNA-binding protein (CIRBP).

Proteasome inhibition increases recruitment of IκB kinase ß (IKKß), S536P-p65, and transcription factor EGR1 to interleukin-8 (IL-8) promoter, resulting in increased IL-8 production in ovarian cancer cells.

Chorismate pyruvate-lyase and 4-hydroxy-3-solanesylbenzoate decarboxylase are required for plastoquinone biosynthesis in the cyanobacterium Synechocystis sp. PCC6803.

Toll-like receptor agonists promote prolonged triglyceride storage in macrophages.

Amino Acids Activate Mammalian Target of Rapamycin (mTOR) Complex 1 without Changing Rag GTPase Guanyl Nucleotide $Charging^{*}$

Increased Ribozyme Activity in Crowded Solutions

The extent of protease resistance of misfolded prion protein is highly dependent on the salt concentration.

Pannexin 3 Inhibits Proliferation of Osteoprogenitor Cells by Regulating Wnt and p21 Signaling

Characterization of a reduced form of plasma plasminogen as the precursor for angiostatin formation.

Conformational stability of mammalian prion protein amyloid fibrils is dictated by a packing polymorphism within the core region.

Mechanisms of allosteric activation and inhibition of the deoxyribonucleoside triphosphate triphosphohydrolase from Enterococcus faecalis.

Intracellular and extracellular carbonic anhydrases cooperate non-enzymatically to enhance activity of monocarboxylate transporters.

The role of prolyl hydroxylase domain protein (PHD) during rosiglitazone-induced adipocyte differentiation.

Nuclear import factor Srp1 and its associated protein Sts1 couple ribosome-bound nascent polypeptides to proteasomes for cotranslational degradation.

Novel piperazine-based compounds inhibit microtubule dynamics and sensitize colon cancer cells to tumor necrosis factor-induced apoptosis.

Thioredoxin-mediated denitrosylation regulates cytokine-induced nuclear factor κB (NF-κB) activation.

NF-κB and enhancer-binding CREB protein scaffolded by CREB-binding protein (CBP)/p300 proteins regulate CD59 protein expression to protect cells from complement attack.

Polo-like kinase 2, a novel ADAM17 signaling component, regulates tumor necrosis factor ectodomain shedding.

Structural basis for allosteric coupling at the membrane-protein interface in Gloeobacter violaceus ligand-gated ion channel (GLIC).

Cardiolipin is dispensable for oxidative phosphorylation and non-fermentative growth of alkaliphilic Bacillus pseudofirmus OF4.

Manipulation of cardiac phosphatidylinositol 3-kinase (PI3K)/Akt signaling by apoptosis regulator through modulating IAP expression (ARIA) regulates cardiomyocyte death during doxorubicin-induced cardiomyopathy.

Control of helicase loading in the coupled DNA replication and recombination systems of bacteriophage T4.

Chronophin dimerization is required for proper positioning of its substrate specificity loop.

Evidence for transceptor function of cellodextrin transporters in Neurospora crassa.

UBE4B protein couples ubiquitination and sorting machineries to enable epidermal growth factor receptor (EGFR) degradation.

CCAAT/enhancer-binding protein ß regulates the repression of type II collagen expression during the differentiation from proliferative to hypertrophic chondrocytes.

Aortic carboxypeptidase-like protein (ACLP) enhances lung myofibroblast differentiation through transforming growth factor ß receptor-dependent and -independent pathways.

Quantitative aspects of cGMP phosphodiesterase activation in carp rods and cones.

Ascorbate efflux as a new strategy for iron reduction and transport in plants.

Rho-GTPase-activating protein interacting with Cdc-42-interacting protein 4 homolog 2 (Rich2): a new Ras-related C3 botulinum toxin substrate 1 (Rac1) GTPase-activating protein that controls dendritic spine morphogenesis.

Host SAMHD1 protein promotes HIV-1 recombination in macrophages.

Role of stearoyl-CoA desaturase-1 in skin integrity and whole body energy balance.

GCN5-like protein 1 (GCN5L1) controls mitochondrial content through coordinated regulation of mitochondrial biogenesis and mitophagy.

HIV-1 Tat- and Vpr-responsive microRNAs of neuronal cells.

Year: 2014 Volume: 289 Issue: 4

Year: 2014 Volume: 289 Issue: 3

Year: 2014 Volume: 289 Issue: 2

Year: 2014 Volume: 289 Issue: 1

Year: 2013 Volume: 288

Year: 2012 Volume: 287

Year: 2011 Volume: 286

Year: 2010 Volume: 285

Year: 2009 Volume: 284

Year: 2008 Volume: 283

Year: 2007 Volume: 282

Year: 2006 Volume: 281

Year: 2005 Volume: 280

Year: 2004 Volume: 279

Year: 2003 Volume: 278

Year: 2002 Volume: 277

Year: 2001 Volume: 276

Year: 2000 Volume: 275

Year: 1999 Volume: 274

Year: 1998 Volume: 273

Year: 1997 Volume: 272

Year: 1996 Volume: 271

Year: 1995 Volume: 270

Year: 1994 Volume: 269

Year: 1993 Volume: 268

Year: 1992 Volume: 267

Year: 1991 Volume: 266

Year: 1990 Volume: 265

Year: 1989 Volume: 264

Year: 1988 Volume: 263

Year: 1987 Volume: 262

Year: 1986 Volume: 261

Year: 1984 Volume: 259

Year: 1982 Volume: 257

Year: 1981 Volume: 256

Year: 1975 Volume: 250

Amino Acids Activate Mammalian Target of Rapamycin (mTOR) Complex 1 without Changing Rag GTPase Guanyl Nucleotide $Charging^{*}$

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Oshiro, Noriko Avruch, Joseph Rapley, Joseph
Description	Author Affiliation: Oshiro N ( From the Department of Molecular Biology and Diabetes Unit, Medical Services, Massachusetts General Hospital, Boston, Massachusetts 02114 and the Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115.)
Abstract	Activation of mammalian target of rapamycin complex 1 (mTORC1) by amino acids is mediated in part by the Rag GTPases, which bind the raptor subunit of mTORC1 in an amino acid-stimulated manner and promote mTORC1 interaction with Rheb-GTP, the immediate activator. Here we examine whether the ability of amino acids to regulate mTORC1 binding to Rag and mTORC1 activation is due to the regulation of Rag guanyl nucleotide charging. Rag heterodimers in vitro exhibit a very rapid, spontaneous exchange of guanyl nucleotides and an inability to hydrolyze GTP. Mutation of the Rag P-loop corresponding to $Ras^{Ser-17}$ abolishes guanyl nucleotide binding. Such a mutation in RagA or RagB inhibits, whereas in RagC or RagD it enhances, Rag heterodimer binding to mTORC1. The binding of wild-type and mutant Rag heterodimers to mTORC1 in vitro parallels that seen with transient expression, but binding to mTORC1 in vitro is entirely independent of Rag guanyl nucleotide charging. HeLa cells stably overexpressing wild-type or P-loop mutant RagC exhibit unaltered amino acid regulation of mTORC1. Despite amino acid-independent raptor binding to Rag, mTORC1 is inhibited by amino acid withdrawal as in parental cells. Rag heterodimers extracted from $^{32}P-labeled$ whole cells, or just from the pool associated with the lysosomal membrane, exhibit constitutive $[^{32}P]GTP$ charging that is unaltered by amino acid withdrawal. Thus, amino acids promote mTORC1 activation without altering Rag GTP charging. Raptor binding to Rag, although necessary, is not sufficient for mTORC1 activation. Additional amino acid-dependent steps couple Rag-mTORC1 to Rheb-GTP.
ISSN	00219258
e-ISSN	1083351X
Journal	Journal of Biological Chemistry
Issue Number	5
Volume Number	289
Language	English
Publisher	American Society for Biochemistry and Molecular Biology (United States)
Publisher Date	2014-01-31
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Amino Acids Metabolism GTP Phosphohydrolases Monomeric GTP-Binding Proteins Multiprotein Complexes TOR Serine-Threonine Kinases Adaptor Proteins, Signal Transducing Dimerization Enzyme Activation Physiology Chemistry Genetics HEK293 Cells HeLa Cells Protein Binding Signal Transduction Tritium Research Support, N.I.H., Extramural Biochemistry Molecular Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in