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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Meoli, Christopher C. Chaudhuri, Rima Murrow, Beverley A. Burchfield, James G. Krycer, James R. James, David E. Stöckli, Jacqueline Prior, Matthew J. Parker, Ben L. Fazakerley, Daniel J. Koumanov, Françoise Holman, Geoffrey D. Thomas, Kristen C. Naghiloo, Sheyda |
| Description | Author Affiliation: Fazakerley DJ ( From the Charles Perkins Centre, School of Molecular Bioscience, and The Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia, and.); Naghiloo S ( From the Charles Perkins Centre, School of Molecular Bioscience, and The Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia, and.); Chaudhuri R ( From the Charles Perkins Centre, School of Molecular Bioscience, and The Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia, and.); Koumanov F ( the Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom.); Burchfield JG ( From the Charles Perkins Centre, School of Molecular Bioscience, and The Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia, and.); Thomas KC ( From the Charles Perkins Centre, School of Molecular Bioscience, and.); Krycer JR ( From the Charles Perkins Centre, School of Molecular Bioscience, and The Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia, and.); Prior MJ ( The Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia, and.); Parker BL ( From the Charles Perkins Centre, School of Molecular Bioscience, and The Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia, and.); Murrow BA ( The Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia, and.); Stöckli J ( From the Charles Perkins Centre, School of Molecular Bioscience, and The Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia, and.); Meoli CC ( From the Charles Perkins Centre, School of Molecular Bioscience, and The Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia, and.); Holman GD ( the Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom.); James DE ( From the Charles Perkins Centre, School of Molecular Bioscience, and The Garvan Institute of Medical Research, Sydney, New South Wales 2010, Australia, and School of Medicine, University of Sydney, Sydney, New South Wales 2006, Australia, david.james@sydney.edu.au.) |
| Abstract | Insulin signaling augments glucose transport by regulating glucose transporter 4 (GLUT4) trafficking from specialized intracellular compartments, termed GLUT4 storage vesicles (GSVs), to the plasma membrane. Proteomic analysis of GSVs by mass spectrometry revealed enrichment of 59 proteins in these vesicles. We measured reduced abundance of 23 of these proteins following insulin stimulation and assigned these as high confidence GSV proteins. These included established GSV proteins such as GLUT4 and insulin-responsive aminopeptidase, as well as six proteins not previously reported to be localized to GSVs. Tumor suppressor candidate 5 (TUSC5) was shown to be a novel GSV protein that underwent a 3.7-fold increase in abundance at the plasma membrane in response to insulin. siRNA-mediated knockdown of TUSC5 decreased insulin-stimulated glucose uptake, although overexpression of TUSC5 had the opposite effect, implicating TUSC5 as a positive regulator of insulin-stimulated glucose transport in adipocytes. Incubation of adipocytes with TNFα caused insulin resistance and a concomitant reduction in TUSC5. Consistent with previous studies, peroxisome proliferator-activated receptor (PPAR) γ agonism reversed TNFα-induced insulin resistance. TUSC5 expression was necessary but insufficient for PPARγ-mediated reversal of insulin resistance. These findings functionally link TUSC5 to GLUT4 trafficking, insulin action, insulin resistance, and PPARγ action in the adipocyte. Further studies are required to establish the exact role of TUSC5 in adipocytes. |
| ISSN | 00219258 |
| e-ISSN | 1083351X |
| Journal | Journal of Biological Chemistry |
| Issue Number | 39 |
| Volume Number | 290 |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology (United States) |
| Publisher Date | 2015-09-25 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Adipocytes Physiology Glucose Transporter Type 4 Metabolism Insulin Proteomics Tumor Suppressor Proteins 3T3-L1 Cells Animals Mice Rats, Wistar Genetics Research Support, Non-U.S. Gov't Biochemistry Molecular Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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