NDLI: DNA damage by sulfite autoxidation catalyzed by cobalt complexes

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Coichev, Nina Alipázaga, María V. Linares, Edlaine Moreno, Ruben G. M. Medeiros, Marisa H. G.
Description	Author Affiliation: Alipázaga MV ( Instituto de Química, Universidade de São Paulo, CP 26077, CEP 05513-970, São Paulo, SP, Brazil. ncoichev@iq.usp.br)
Abstract	DNA damage was investigated in the presence of sulfite , dissolved oxygen and cobalt( II) complexes with glycylglycylhistidine , glycylhistidyllysine , glycylglycyltyrosylarginine and tetraglycine . These studies indicated that only Co( II) complexed with glycylglycylhistidine ( GGH ) induced DNA strand breaks at low sulfite concentrations (1–80 μM) via strong oxidants formed in the reaction. In the presence of the other complexes, some damage occurred only in the presence of high sulfite concentrations (0.1–2.0 mM) after incubation for 4 h. In the presence of GGH , Co( II) and dissolved $O_{2},$ DNA damage must involve a reactive high-valent cobalt complex. The damaging effect was increased by adding S( IV) , due to the oxysulfur radicals formed as intermediates in S( IV) autoxidation catalyzed by the complex. $SO_{3}˙^{−},$ HO˙ and H˙ radicals were detected by EPR-spin trapping experiments with DMPO ( 5,5-dimethyl-1-pyrroline N-oxide ). The results indicate that Co( II) binds $O_{2}$ in the presence of GGH , and leads to the formation of a DMPO – HO˙ adduct without first forming free superoxide or hydroxyl radical, supporting the participation of a reactive high-valent cobalt complex.
ISSN	14779226
Issue Number	41
e-ISSN	13645447
Journal	Dalton Trans.
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2008-11-07
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Cobalt Chemistry DNA Damage Drug Effects Organometallic Compounds Metabolism Oxygen Sulfites Pharmacology Amino Acid Sequence Catalysis Copper Electron Spin Resonance Spectroscopy Electrophoresis, Agar Gel Free Radicals Oligopeptides Spectrophotometry, Ultraviolet Journal Article Research Support, Non-U.S. Gov't
Content Type	Text
Resource Type	Article
Subject	Inorganic Chemistry

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