NDLI: Adsorption and spectroscopic characterization of lactoferrin on hydroxyapatite nanocrystals

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Di Foggia, Michele Iafisco, Michele Roveri, Norberto Bonora, Sergio Prat, Maria
Description	Author Affiliation: Iafisco M ( Dipartimento di Chimica G. Ciamician, Alma Mater Studiorum Università di Bologna, Via Selmi 2, 40126, Bologna, Italy.)
Abstract	Lactoferrin (LF), a well-characterized protein of blood plasma and milk with antioxidant , cariostatic, anticarcinogenic and anti-inflammatory properties, has been adsorbed onto biomimetic hydroxyapatite (HA) nanocrystals at two different pH values (7.4 and 9.0). The interaction was herein investigated by spectroscopic, thermal and microscopic techniques. The positive electrostatic surface potential of LF at pH 7.4 allows a strong surface interaction with the slightly negative HA nanocrystals and avoids the protein – protein interaction, leading to the formation of a coating protein monolayer. In contrast, at pH 9.0 the surface potential of LF is a mix of negative and positive zones favouring the protein – protein interaction and reducing the interaction with HA nanocrystals ; as a result a double layer of coating protein was formed. These experimental findings are supported by the good fittings of the adsorption isotherms by different theoretical models according to Langmuir, Freundlich and Langmuir–Freundlich models. The nanosized HA does not appreciably affect the conformation of the adsorbed protein . In fact, using FT-Raman and FT-IR , we found that after adsorption the protein was only slightly unfolded with a small fraction of the α-helix structure being converted into turn, while the β-sheet content remained almost unchanged. The bioactive surface of HA functionalized with LF could be utilized to improve the material performance towards the biological environment for biomedical applications.
ISSN	14779226
Issue Number	4
Volume Number	40
e-ISSN	13645447
Journal	Dalton Trans.
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2011-01-28
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Durapatite Chemistry Lactoferrin Nanoparticles Adsorption Biomimetic Materials Hydrogen-Ion Concentration Spectroscopy, Fourier Transform Infrared Spectrum Analysis, Raman Static Electricity Journal Article Research Support, Non-U.S. Gov't
Content Type	Text
Resource Type	Article
Subject	Inorganic Chemistry

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