NDLI: Light induced photoreactions with plasmid DNA by Cu / Ru and Cu / Ru / Part multi-metallic porphyrins

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Swavey, Shawn Xu, Zhiming
Description	Author Affiliation: Xu Z ( Department of Chemistry, University of Dayton, 300 College Park, Dayton, OH 45469-2357, USA.)
Abstract	Coordination of two [Ru(bipy) $_{2}Cl]$ $^{+}$ moieties (where bipy = 2,2′-bipyridine ) to the pyridyl nitrogens in the 5,10-positions of meso-5,10,15-(4-Pyridyl)-20-(pentafluorophenyl)porphyrin gives the diruthenium porphyrin complex II. Insertion of copper( II) into the porphyrin center allows for the third pyridyl nitrogen to coordinate to Pt(dmso)Cl $_{2}$ . Electronic transitions associated with the ruthenium porphyrin include an intense Soret band and four less intense Q-bands in the visible region of the spectrum . An intense π–π* transition in the UV region associated with the bipyridyl groups and a metal to ligand charge transfer (MLCT) band appearing as a shoulder to the Soret band are also observed. A slight blue shift of the Soret band and collapse of the Q-bands into one band is observed upon insertion of Cu( II) into the porphyrin center. No change in the electronic spectrum is observed upon coordination of the Pt(dmso)Cl $_{2}$ moiety. Electrochemical properties associated with the complexes include a redox couple in the cathodic region attributed to the porphyrin and a redox couple in the anodic region due to the Ru $^{III}$ $^{/II}$ couple. DNA titrations of the Cu / Ru and Cu / Ru / Pt porphyrins indicate that both complexes interact strongly with DNA potentially through a partial intercalation mechanism. Gel electrophoresis studies indicate that the Cu / Ru / Pt porphyrin has a greater effect on DNA migration through the gel than the well known DNA binding agent cis-platin . Irradiation of aqueous solutions of the Cu / Ru porphyrin and supercoiled DNA at a 5:1 base pair to complex ratio (in the absence of oxygen ) with visible light above 400 nm shows a nicking of the DNA . Repeat experiments in the presence of oxygen show that the Cu / Ru porphyrin photocleaves the DNA , giving the linear form, as evidenced by gel electrophoresis .
ISSN	14779226
Issue Number	28
Volume Number	40
e-ISSN	13645447
Journal	Dalton Trans.
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2011-07-28
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Copper Chemistry DNA Light Plasmids Genetics Platinum Porphyrins Ruthenium DNA, Superhelical Photochemistry Journal Article
Content Type	Text
Resource Type	Article
Subject	Inorganic Chemistry

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