NDLI: Towards cancer cell-specific phototoxic organometallic rhenium(I) complexes

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Heier, Jakob Gasser, Gilles Leonidova, Anna Pierroz, Vanessa Rubbiani, Riccardo Ferrari, Stefano
Description	Author Affiliation: Leonidova A ( Institute of Inorganic Chemistry, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland. gilles.gasser@aci.uzh.ch.)
Abstract	Over the recent years, several Re(I) organometallic compounds have been shown to be toxic to various cancer cell lines. However, these compounds lacked sufficient selectivity towards cancer tissues to be used as novel chemotherapeutic agents. In this study, we probe the potential of two known N,N-bis(quinolinoyl) Re(I) tricarbonyl complex derivatives, namely Re(I) tricarbonyl [N,N-bis(quinolin-2-ylmethyl)amino]-4-butane-1-amine $(Re–NH_{2})$ and Re(I) tricarbonyl [N,N-bis(quinolin-2-ylmethyl)amino]-5-valeric acid (Re–COOH), as photodynamic therapy (PDT) photosensitizers. $Re–NH_{2}$ and Re–COOH proved to be excellent singlet oxygen generators in a lipophilic environment with quantum yields of about 75%. Furthermore, we envisaged to improve the selectivity of Re–COOHvia conjugation to two types of peptides, namely a nuclear localization signal (NLS) and a derivative of the neuropeptide bombesin, to form Re–NLS and Re–Bombesin, respectively. Fluorescent microscopy on cervical cancer cells (HeLa) showed that the conjugation of Re–COOH to NLS significantly enhanced the compound's accumulation into the cell nucleus and more specifically into its nucleoli. Importantly, in view of PDT applications, the cytotoxicity of the Re complexes and their bioconjugates increased significantly upon light irradiation. In particular, Re–Bombesin was found to be at least 20-fold more toxic after light irradiation. DNA photo-cleavage studies demonstrated that all compounds damaged DNA via singlet oxygen and, to a minor extent, superoxide production.
ISSN	14779226
Issue Number	11
Volume Number	43
e-ISSN	13645447
Journal	Dalton Trans.
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2014-03-21
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Organometallic Compounds Chemistry Pharmacology Photosensitizing Agents Rhenium Bombesin Cell Line Cell Survival Drug Effects Radiation Effects Drug Delivery Systems HeLa Cells Humans Neoplasms Drug Therapy Nuclear Localization Signals Administration & Dosage Pharmacokinetics Singlet Oxygen Metabolism Journal Article Research Support, Non-U.S. Gov't
Content Type	Text
Resource Type	Article
Subject	Inorganic Chemistry

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